[Histonet] RE: Metal molds

Wed Oct 10 08:52:23 CDT 2012

We clean ours in the VIP every Friday. We recycle our xylene substitute
from the VIP but you cannot recycle the cleaning alcohol.

They are all "bright and shiny" for Monday morning!

Jen Campbell

On Wed, Oct 10, 2012 at 9:46 AM, Bartlett, Jeanine (CDC/OID/NCEZID) <
jqb7 <@t> cdc.gov> wrote:

> Does anyone that uses the VIP to clean their base molds also recycle the
> alcohols/xylene?  We were told that you should not clean the molds in the
> processor if you were recycling. Thanks!
>
> Jeanine H. Bartlett
> Centers for Disease Control and Prevention
> Infectious Diseases Pathology Branch
> 404-639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Harris, Diana
> Sent: Wednesday, October 10, 2012 9:42 AM
> To: 'Susan.Walzer <@t> HCAHealthcare.com'; joelleweaver <@t> hotmail.com;
> valerie.hannen <@t> parrishmed.com
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: Metal molds
>
> At our lab we clean the molds in the VIP cleaning cycle then dip in mold
> release also.  Works well.  The only precaution is to drain the molds well
> after dipping otherwise embedding can be effected.
>
>
> Diana Harris
> QC & Method Development Technologist
> Dept. Of Laboratory Medicine
> Anatomical Pathology
> Royal Jubilee Hospital
> Victoria, BC Canada
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Susan.Walzer <@t> HCAHealthcare.com
> Sent: Wednesday, October 10, 2012 12:01 AM
> To: joelleweaver <@t> hotmail.com; valerie.hannen <@t> parrishmed.com
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: Metal molds
>
> We put our molds in the VIP before running the cleaning cycle daily. Then
> we dip them in alcohol containing mold release..air dry and store.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of joelle weaver
> Sent: Tuesday, October 09, 2012 3:27 PM
> To: valerie.hannen <@t> parrishmed.com
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: Metal molds
>
>
> I always cleaned them daily, either the very hot water, soapy water
> method, with water running over them in the sink with them on their sides
> so it passes over them, not upright so the water sits in them- then a rinse
> in alcohol and completely air dry. Or you can always do the clean cycle
> with the racks, running them through xylene, etc. They come out very clean
> this way- used an old processor that was a backup for this most of the
> time. But I always did them daily, but also wiped each one out with gauze
> if I used them twice in an embedding session ( for more than one specimen
> in that large batch). Also I like metal, I hate those plastic ones. If you
> keep the block face surface of the mold warm-hot,  and flatten before it
> turns completely white the specimen is at the surface and you are able to
> see the edges easily without a lot of "facing". I think this saves time
> cutting through paraffin, and saves blades. Plus if the specimen is not
> flat enough, you see it right away and know if you must re-embed to get a
> complete, representative section, rather than after you have cut some
> superficial parts of some edges away and not others, only to have to
> re-embed anyhow. The other problems I see are when people are "afraid" of
> big molds- please if you are only taking one section, use one large enough
> to leave a perimeter. Don't try to squeeze it into a medium mold, you are
> unlikely to need multiple sections on one slide and it is much easier to
> get flat and get a good section.  Also please  put enough paraffin on top,
> so that when it is cool the layer over the grooves in the cassette is not
> so thin that you can clearly see the depressions. That little bit of
> paraffin is much cheaper than tech time in re-embedding and fussing with a
> block longer than you should.  Not so much a big issue for many specimens,
> but anything hard/ dense, such as bone, cervix, uterus, leeps, ( you get
> the idea) it is not anchored enough without a good dose of paraffin,
> causing more chatter when you section, and maybe chipping out more
> frequently, or even the whole bottom surface to lift off the cassette. I
> guess I have some "pet peeves" with this topic,  so thanks for letting me
> get that out!
>
>
>
>
> Joelle Weaver MAOM, HTL (ASCP) QIHC
>  > From: Valerie.Hannen <@t> parrishmed.com
> > To: billodonnell <@t> catholichealth.net; histonet <@t> lists.utsouthwestern.edu
> > Date: Tue, 9 Oct 2012 10:51:01 -0400
> > CC:
> > Subject: [Histonet] RE: Metal molds
> >
> > We clean our molds once a week. Soak them in Xylene to remove paraffin,
> soak in 100% alcohol to remove xylene, rinse in running water, dry and
> spray with mold release solution.
> >
> > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief
> > Parrish Medical Center
> > 951 N. Washington Ave.
> > Titusville, Florida 32976
> > Phone:(321) 268-6333 ext. 7506
> > Fax: (321) 268-6149
> > valerie.hannen <@t> parrishmed.com
> >
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> > O'Donnell, Bill
> > Sent: Monday, October 08, 2012 4:32 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] Metal molds
> >
> >
> >  OK folks, I know I should be smarter than this and I haven't seen
> discussion on it lately....
> >
> > Are people cleaning their metal embedding molds after evey embedding
> session?
> >
> > If not, how often do you clean them?
> >
> > Do you clean them at all?
> >
> > If you clean them, how do you do it?
> >
> > Thanks
> >
> > Bill
> > William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good
> > Samaritan Hospital 10 East 31st Street Kearney, NE 68847
> >
> > SERENITY is not freedom from the storm, but peace amid the storm.
> >
> > Cultivate it in PRAYER!
> >
> >
> >
> >
> > This electronic mail and any attached documents are intended solely for
> the named addressee(s) and contain confidential information. If you are not
> an addressee, or responsible for delivering this email to an addressee, you
> have received this email in error and are notified that reading, copying,
> or disclosing this email is prohibited. If you received this email in
> error, immediately reply to the sender and delete the message completely
> from your computer system.
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > =============
> > "This email is intended solely for the use of the individual to whom
> > it is addressed and may contain information that is privileged,
> > confidential or otherwise exempt from disclosure under applicable law.
> > If the reader of this email is not the intended recipient or the
> > employee or agent responsible for delivering the message to the
> > intended recipient, you are hereby notified that any dissemination,
> > distribution, or copying of this communication is strictly prohibited.
> > If you have received this communication in error, please immediately
> > delete this message. Thank you"
> > =============
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

-- 
Jen Campbell, HT(ASCP)
Supervisor of Technical Services
Muhlbauer Dermatopathology Laboratory
61 Monroe Avenue, Ste B
Pittsford NY 14534
P: 585.586.5166
F: 585.586.3137

IMPORTANT NOTICE:  This e-mail and any attachments may contain confidential
or sensitive information which is, or may be, legally privileged or
otherwise protected by law from further disclosure.  It is intended only
for the addressee.  If you received this in error or from someone who was
not authorized to send it to you, please do not distribute, copy or use it
or any attachments.  Please notify the sender immediately by reply e-mail
and delete this from your system. Thank you for your cooperation.