[Histonet] RE: Histonet Digest, Vol 107, Issue 13 Prostate grossing

Wellen, Terrence D. :LPH Lab TWELLEN <@t> LHS.ORG
Tue Oct 9 12:11:43 CDT 2012


 

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Sent: Tue 10/9/2012 10:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 107, Issue 13



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Today's Topics:

   1. Cellient (Schofield, Kevin)
   2. RE: Flourescent mounting medium- PLEASE HELP!!! (Entwistle, Laura)
   3. RE: Metal molds (Goins, Tresa)
   4. prostate trimming protocol (Contact HistoCare)
   5. New Reagent Lot Verification (Amy Self)
   6. Re: prostate trimming protocol (Rene J Buesa)
   7. RE: RE: Mitochondiral membrane (Patsy Ruegg)
   8. RE: prostate trimming protocol (WILLIAM DESALVO)


----------------------------------------------------------------------

Message: 1
Date: Tue, 9 Oct 2012 15:00:37 +0000
From: "Schofield, Kevin" <kevin.schofield <@t> yale.edu>
Subject: [Histonet] Cellient
To: "Histonet <@t> lists.utsouthwestern.edu"
        <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1C71B9CD502B094FA755E48906457C8F0938FE5F <@t> x10-mbx3.yu.yale.edu>
Content-Type: text/plain; charset="us-ascii"

I am curious if there are any instituions that are using the Cellient
instrument for cell blocks on ECC cases that are scant or any surgical
specimen.
--
Kevin Schofield BS CT(ASCP)
Director, Outreach Clinical Operations
Yale University
Department of Pathology
(P) 203-737-2928
(F) 203-785-2641



>




------------------------------

Message: 2
Date: Tue, 9 Oct 2012 15:22:43 +0000
From: "Entwistle, Laura" <lentwistle <@t> ucsd.edu>
Subject: RE: [Histonet] Flourescent mounting medium- PLEASE HELP!!!
To: Jonathan Cremer <Jonathan.Cremer <@t> med.kuleuven.be>, Candice Smoots
        <candice_camille <@t> yahoo.com>, Histonet
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <A65C72FCD532314C9AAFCC11BF5DF5DD209815F8 <@t> XMAIL-MBX-BH1.AD.UCSD.EDU>
Content-Type: text/plain; charset="us-ascii"

We use this medium.  We have found that if you shake it before use it becomes a little more viscous, but toss it if it becomes too gel like.  We also allow our sections to dry, but keep them in the dark as much as possible.  You also need to be sure that you are using coverglass that works with fluorescent material.

Laura

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jonathan Cremer
Sent: Monday, October 08, 2012 7:20 AM
To: Candice Smoots; Histonet
Subject: RE: [Histonet] Flourescent mounting medium- PLEASE HELP!!!

I have no experience with this particular mounting medium, but from what I gather it is like any other aqueous mounting medium. You should blot your slides to remove as much water as possible, without letting your sections dry. Then apply a small amount of mounting medium to the slide or coverslip (depending on preference) and mount.
Apparently it solidifies at the edge, so no need to seal with nail polish.

If your bottle has solidified to the point where it is a gel instead of a viscous liquid, you can toss it. (unless the datasheet specifies otherwise...)

Jonathan

________________________________________
Van: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] namens Candice Smoots [candice_camille <@t> yahoo.com]
Verzonden: maandag 8 oktober 2012 15:42
To: Histonet
Onderwerp: [Histonet] Flourescent mounting medium- PLEASE HELP!!!

Hi Histonet


In our lab, we are trying to mount our flourescent stained sections with polyvinyl alcohol mounting medium with DABCO. WE are unsure if it is to be mounted while the sections are wet or do we let me air dry.

Another question is... How to actually use it. It looks to be a little solidified. Kind of like jello. Is this normal? do I dilute it?  Please Please Help!

I remain yours truely,

Candice Camille
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------------------------------

Message: 3
Date: Tue, 9 Oct 2012 15:49:13 +0000
From: "Goins, Tresa" <TGoins <@t> mt.gov>
Subject: [Histonet] RE: Metal molds
To: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <CA4DF32ED505D94BB55E95487D8E984130847E8C <@t> DOAISD5205.state.mt.ads>
Content-Type: text/plain; charset="us-ascii"

We haven't cleaned our molds for five years - if the blocks are cold enough after embedding, there is no problem removing the block. 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Monday, October 08, 2012 2:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Metal molds


 OK folks, I know I should be smarter than this and I haven't seen discussion on it lately....

Are people cleaning their metal embedding molds after evey embedding session?

If not, how often do you clean them?

Do you clean them at all?

If you clean them, how do you do it?

Thanks

Bill
William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.

Cultivate it in PRAYER!




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------------------------------

Message: 4
Date: Tue, 9 Oct 2012 10:53:37 -0500
From: Contact HistoCare <contact <@t> histocare.com>
Subject: [Histonet] prostate trimming protocol
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <F679BD4F-861D-406D-B8DE-828E89925D0C <@t> histocare.com>
Content-Type: text/plain;       charset=us-ascii

Good morning all,

Could someone with more knowledge in this matter than I have help shed a little light?

While at a nationally-renowned medical facility, I've come across something rather interesting (to me) for which no one in the immediate lab has a definitive answer for.

I see varying trimming(or grossing) techniques by the residents. I'm told that it's very common to have poorly grossed tissue submitted regularly whenever a new group comes through, but nothing is done to correct it.

It runs the gamut from non-decalcified bone, or humongous chunks of tissue that barely fits in the cassette but has to be nearly shoved into the mold, and tissue that's >5mm, seriously.

This time, it's prostate tissue. I've been places where maybe 3 or 4 sections were submitted from the area of interest and maybe a sample of normal tissue just for differentiation. But here, it's common to receive anywhere from 30 to 50 cassettes from the same site. I'm guessing they don't want to discard ANY tissue.

What's interesting is some of this is submitted as a bunch of very tiny slivers in some cassettes and then nickel and quarter-sized chunks from the same site in others.

Has anyone else seen prostate submitted this way? Is there a rhyme or reason that I'm not aware of?

Thanks

M


www.HistoCare.com


------------------------------

Message: 5
Date: Tue, 9 Oct 2012 11:53:24 -0400
From: Amy Self <ASelf <@t> georgetownhospitalsystem.org>
Subject: [Histonet] New Reagent Lot Verification
To: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <EEED2FF216617D45AFF64BF53EC4F9C75CA8B23967 <@t> GMHDTCEXCH.gmhpost.com>
Content-Type: text/plain; charset="us-ascii"

Does anyone have a Lot to Lot Verification QC form or procedure that they would be willing to share with me for use with the Ventana Benchmark when comparing lot to lots of antibodies and detection kits?

Thanks in advance for your help, Amy


Georgetown Hospital System
843-527-7179
NOTE:
 The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer.
Thank you.


------------------------------

Message: 6
Date: Tue, 9 Oct 2012 09:04:37 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] prostate trimming protocol
To: Contact HistoCare <contact <@t> histocare.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1349798677.87222.YahooMailNeo <@t> web163105.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Yes, and that depends, as you note, on the residents doing the grossing.
Your solution: get the pathologists involved and find out how they want the grossing to be done, and make sure that the pathologists communicate their preferences to the residents.
It may be that the ones wanting to "see everything" are the pathologists. Involve the pathologists in your concerns.
René J.


________________________________
From: Contact HistoCare <contact <@t> histocare.com>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, October 9, 2012 11:53 AM
Subject: [Histonet] prostate trimming protocol

Good morning all,

Could someone with more knowledge in this matter than I have help shed a little light?

While at a nationally-renowned medical facility, I've come across something rather interesting (to me) for which no one in the immediate lab has a definitive answer for.

I see varying trimming(or grossing) techniques by the residents. I'm told that it's very common to have poorly grossed tissue submitted regularly whenever a new group comes through, but nothing is done to correct it.

It runs the gamut from non-decalcified bone, or humongous chunks of tissue that barely fits in the cassette but has to be nearly shoved into the mold, and tissue that's >5mm, seriously.

This time, it's prostate tissue. I've been places where maybe 3 or 4 sections were submitted from the area of interest and maybe a sample of normal tissue just for differentiation. But here, it's common to receive anywhere from 30 to 50 cassettes from the same site. I'm guessing they don't want to discard ANY tissue.

What's interesting is some of this is submitted as a bunch of very tiny slivers in some cassettes and then nickel and quarter-sized chunks from the same site in others.

Has anyone else seen prostate submitted this way? Is there a rhyme or reason that I'm not aware of?

Thanks

M


http://www.histocare.com/
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------------------------------

Message: 7
Date: Tue, 9 Oct 2012 10:06:41 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] RE: Mitochondiral membrane
To: "'Elizabeth Chlipala'" <liz <@t> premierlab.com>, <fujisaws <@t> mskcc.org>,
        <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <978750B890A648CFB60B1403498482F3 <@t> DESKTOP3>
Content-Type: text/plain;       charset="us-ascii"

I have use a mitochondria marker from Dako but it is cytoplasmic not on the
membrane.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pruegg <@t> ihctech.net
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Tuesday, October 09, 2012 8:55 AM
To: 'fujisaws <@t> mskcc.org'; Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Mitochondiral membrane

Sho



There is a mitochondria marker for human tissue samples they are from Novus,
not sure if it's a membrane marker or not.  Good Luck!


Protocol:  Mouse anti Mitochondria
Clone: MTC02
Vendor: Novus Biologicals
Catalog Number: NB600-556
Species: Mouse
Isotype: IgG21

Spec Sheet: Novus
Mito<http://www.novusbio.com/data_sheet/index/NB600-556&prev_module=search>



Liz



Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, CO 80308-1592

(303) 682-3949 office

(303) 682-9060 fax

(303) 881-0763 cell

www.premierlab.com



Ship to address:



1567 Skyway Drive, Unit E

Longmont, CO 80504



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
fujisaws <@t> mskcc.org
Sent: Tuesday, October 09, 2012 8:43 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mitochondiral membrane



Dear all-



I have a user looking to label mitochondrial membrane.



Can anybody recommend a commercial antibody? I'm guessing one of the TOM
complex proteins would be a good target.



Thank you in advance.





Sho Fujisawa

Molecular Cytology Core Facility

Memorial Sloan-Kettering Cancer Center

415 East 68th St. ZRC-1840

New York, NY 10065

(646) 888-2186, x2186







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------------------------------

Message: 8
Date: Tue, 9 Oct 2012 10:11:37 -0600
From: WILLIAM DESALVO <wdesalvo.cac <@t> outlook.com>
Subject: RE: [Histonet] prostate trimming protocol
To: Contact HistoCare <contact <@t> histocare.com>, histonet
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY002-W10314C7D9DBB9DFA700799E828F0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Talk w/ the pathologists or residents, have a conversation about what and why. The multiple samples could be due to teaching or cancer protocols. The number, size and selection area of samples is always the responsibility of the pathologists. I find it best to not wonder why, just ask. Typically the pathologist will be more than happy to discuss and explain their dissection and sampling protocol.

The variation in thickness and sample size by residents can be corrected by the trainer, typically a PA or pathologist. There may be a need to rewrite or create an good standardized procedure that the trainner can use. Again, have a discussion about how consistency in sample size, crisp edges and consistent thickness, < 3 mm, can improve quality. I suggest you start w/ a visual, a good old nickel. Consider super gluing one to every gross dissection board. Precision at the grossing board leads to increased quality in processing, microtomy and staining.

I am firm believer that if you concentrate on quality, both in action and conversation, you will get more bang for your buck! Always remember, when you start asking the questions, be prepared to get involved w/ training and correcting the process.




William DeSalvo, B.S., HTL(ASCP)
Production Manager-Anatomic Pathology
Chair, NSH Quality Control Committee
Owner/Consultant, Collaborative Advantage Consulting



> From: contact <@t> histocare.com
> Date: Tue, 9 Oct 2012 10:53:37 -0500
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] prostate trimming protocol
>
> Good morning all,
>
> Could someone with more knowledge in this matter than I have help shed a little light?
>
> While at a nationally-renowned medical facility, I've come across something rather interesting (to me) for which no one in the immediate lab has a definitive answer for.
>
> I see varying trimming(or grossing) techniques by the residents. I'm told that it's very common to have poorly grossed tissue submitted regularly whenever a new group comes through, but nothing is done to correct it.
>
> It runs the gamut from non-decalcified bone, or humongous chunks of tissue that barely fits in the cassette but has to be nearly shoved into the mold, and tissue that's >5mm, seriously.
>
> This time, it's prostate tissue. I've been places where maybe 3 or 4 sections were submitted from the area of interest and maybe a sample of normal tissue just for differentiation. But here, it's common to receive anywhere from 30 to 50 cassettes from the same site. I'm guessing they don't want to discard ANY tissue.
>
> What's interesting is some of this is submitted as a bunch of very tiny slivers in some cassettes and then nickel and quarter-sized chunks from the same site in others.
>
> Has anyone else seen prostate submitted this way? Is there a rhyme or reason that I'm not aware of?
>
> Thanks
>
> M
>
>
> www.HistoCare.com
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
                                         

------------------------------

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