[Histonet] RE: CK AE1/AE3

Goins, Tresa TGoins <@t> mt.gov
Thu Oct 4 13:59:00 CDT 2012

We (veterinary lab) use Pan Cytokeratin Plus [AE1/AE3+8/18] from Biocare.  Enzymatic retrieval will yield positive results for skin from all species tested but HIER using EDTA pH 8 is required for positive results on adenocarcinomas and squamous cell carcinomas.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taylor, Jean
Sent: Wednesday, October 03, 2012 1:47 PM
To: 'ihcrg <@t> googlegroups.com'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] CK AE1/AE3 

Hi Everyone,

I'd like some input as to what labs are using as a procedure for this antibody. Is enzyme digestion or a form of antigen retrieval with heat used? What dilution and detection system do you use as well as what type of machine is it run on?

Thanks for the info,

Jean Taylor, HT (ASCP) QIHC
IHC Tech
Meriter Health Services
Madison, WI
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