[Histonet] Amylase Digestion for glycogen

[Jennifer MacDonald]

We also had problems with diastase digestion.  We researched amylase and 
found it to be REALLY expensive. We found a reasonably priced source with 
American Master Tech.  
(www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=AHDIA50
).  We tried it this semester and the students obtained really good 
results, some better than the dreaded saliva.  I have the PDF for the 
procedure if you would like it. 

Jennifer MacDonald
Mt. San Antonio College




"Peterson, Dan" <1dpeterson <@t> meriter.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
10/03/2012 11:44 AM

To
"'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Amylase Digestion for glycogen






Amy,
I was wondering how you made out with Amylase vs. diastase for your 
glycogen digestion.
Can you send me your protocol if it worked out? We're all of a sudden not 
getting our malt diastase to work.
Thanks
Dan

Daniel R Peterson HT(ASCP)
Histopathology Section Head
Meriter Laboratories
(608) 417-6557
fax (608) 417-6343
1dpeterson <@t> meriter.com

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