[Histonet] Re: [IHCRG] RE: CK AE1/AE3

[ancillarypath <@t> mac.com]

We do not use AE1/AE3 anymore. OSCAR single clone is a much better antibody. We retired AE1/AE3 out of fear that it will lead to false negative results in sentinel nodes.

Hadi



On Oct 3, 2012, at 3:50 PM, Jackie Ferracone wrote:

> Nice antibody, we use Proteinase-K, at a dilution of 1:500. Detection is with DAKO’s ENV+ , and we use a DAKO autostainer.
>  
> Jacqueline D. Ferracone
> Research Associate, Pathobiology
> University of Pennsylvania/New Bolton Center
> Veterinary Medicine
> 382 West Street Road
> Kennett Square, PA, 19348-1692
> 610-925-6391
> 610-350-8860 (cell)
> 610-925-6810 (fax)
>  
> From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of Taylor, Jean
> Sent: Wednesday, October 03, 2012 3:47 PM
> To: 'ihcrg <@t> googlegroups.com'; 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [IHCRG] CK AE1/AE3
>  
> Hi Everyone,
>  
> I’d like some input as to what labs are using as a procedure for this antibody. Is enzyme digestion or a form of antigen retrieval with heat used? What dilution and detection system do you use as well as what type of machine is it run on?
>  
> Thanks for the info,
>  
> Jean Taylor, HT (ASCP) QIHC
> IHC Tech
> Meriter Health Services
> Madison, WI
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