[Histonet] Re: Histonet Digest, Vol 108, Issue 34

[Madeleine Huey]

On Wed, Nov 28, 2012 at 7:10 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Kim Merriam [kmerriam2003 <@t> yahoo.com]
> Sent: Wednesday, November 28, 2012 7:54 AM
> To: Histonet
> Subject: [Histonet] eosin in the processor
>
> Hi Everyone,
>
> Years ago, my lab used to put eosin in the processor to lightly tint the smaller mouse tissues.  I can't remember which station we put it in (I think it was the 2nd 100% ethanol).  Also, back then my lab didn't do any IHC; will the eosin affect any IHC that might be done (I am guessing no, but I want to be sure).
>
> Thanks,
> Kim
>
> Kim Merriam, MA, HT(ASCP)QIHC
> Cambridge, MA

Hello Kim,
I always put the Eosin in the last two 100% alcohol.  The whole
concepts is to have your tissues pick up the Eosin and not wash out
during the processing. Therefore if you put them in the beginning
(ie.70%, 85%.... ) and not the 100% alcohol, they will eventually be
wash out.
Madeleine Huey BS, HTL & QIHC (ASCP)
Supervisor - Pathology (IPOX & Histology)
madeleine_h <@t> elcaminohospital.org