[Histonet] Validation of a new tissue processor

Helen Fedor hfedor <@t> jhmi.edu
Wed Nov 28 15:50:30 CST 2012


You will also need to validate all of the IHC stains and special stains that you do on these tissues. You can take samples from each tissue type and run them in parallel on both machines. Then test all of your special stains and antibodies that you have in your inventory.

:)
Helen

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, November 28, 2012 4:47 PM
To: Howe, Helena; 'histonet <@t> lists.utsouthwestern.edu'
Subject: Re: [Histonet] Validation of a new tissue processor

Using at least 25 different types of tissues, prepare parallel almost identical pairs of slices for each tissue and process them simultaneously in your new tissue processor and in the old one.
Prepare H&E sections and give them to the pathologists concealing the used tissue processor. The pathologists should decide if there are differences between the pairs of sections.
The pathologist's opinion should be registered in the QC file and that will be your validation.
René J.

From: "Howe, Helena" <Helena.Howe <@t> ProMedica.org>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Wednesday, November 28, 2012 2:57 PM
Subject: [Histonet] Validation of a new tissue processor

Does anyone know what the CAP is requiring to validate a new tissue processor?
Thank You
Helena Howe
ProMedica Laboratories
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