We always put a small (tiny!) drop of Kodak PhotoFlo in the water bath. It's a surfactant, so likely any diluted detergent would work. Be careful not to get too much in there, your ribbons will want to sink. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752