[Histonet] procedure for destaining a cytology slide followed by
restaining with a special stain, such as PAS?
Harrison, Sandra C.
Sandra.Harrison3 <@t> va.gov
Fri Nov 16 09:54:55 CST 2012
Does anyone have a procedure for restaining a cytology slide? We
experienced a loss of cells on an FNA aspirate of a lymph node, which
had been originally stained with Diff Qwik. The procedure we used was
1) Destain using 1% Acid Alcohol (1 ml. concentrated HCL added to
99 ml. 70% alcohol) for approx. 30-60 seconds.
2) Restain on automated stainer (Ventana) with PAS kit.
Sandy C. Harrison, HTL (ASCP)
More information about the Histonet