[Histonet] Auramine O Rhodamine B for acid fast bacteria

[Rene J Buesa]

Auramine O is designed to view the slides with fluorescent light and as in any such procedure the slides are not stable and cannot be stored indefinitely.
On the other hand it is a very simple procedure and if your microscope objectives are good and the light source adequate it can render better results than Ziehl-Nielsen because it can demonstrate low level infections (only few organisms) better.
René J.


________________________________
From: "Oneil, Beth Ann" <oneilb <@t> wvuhealthcare.com>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Thursday, November 15, 2012 2:07 PM
Subject: [Histonet] Auramine O Rhodamine B for acid fast bacteria

We are bringing the Auramine O Rhodamine B stain for Acid Fast Bacteria in house very soon.  My question is can you store the slides indefinitely?  With our  immunofluororesce testing on kidney biopsies, the slides are not stable.  I have not seen any literature in regards to the stability of the Auramine-Rhodamine stain.

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor/Technical Specialist
West Virginia University Hospitals
304-293-7629 (office)
304-293-6014 (lab)




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