[Histonet] RE: Histonet Digest, Vol 108, Issue 19 H&E staining

[Steve McClain]

Eosin tissue affinity vs hema is far different with staining times measured in seconds for Eosin vs minutes for Hema.
Overstained Dark eosin makes H appear too light by contrast.
If one considers an analogy of a balance or seesaw with H on one end and E on the other, your solution is obvious.
When H balances E staining appears optimally dark.

Subtracting  eosin to properly balance hema makes whole stain appear more vibrant.

A vivid triple eosin- Treosin is available commercially from Statlab, (satisfied customer- no financial ties)
Eosin Y and Orange G and  acid fuchsin offer differential staining of collagen, fibrin, muscle, cornified cells, especially good for eosinophils and RBCs.

Steve
Steve A. McClain, MD
McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000