[Histonet] RE: holes in tissue sections

[Lynette Pavelich]

Hi Albert,
The first thing that comes to mind to me is aggressive "facing" or rough cutting of the block before taking your final good section. To solve this, after facing the block, (usually at 20+ microns), I go down to my desired slide micron (4) and finish facing at the lower micron for 5-10 more times. This will remove the holes caused 
by the high microns.

Hope this helps,
Lynette

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Santiago, Albert [Albert.Santiago <@t> uphs.upenn.edu]
Sent: Wednesday, November 14, 2012 1:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: holes in tissue sections

From: Santiago, Albert
Sent: Wednesday, November 14, 2012 1:35 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: holes in tissue sections



Hello fellow histonetters, We are a derm lab and we are having slide
quality issues, I am hoping someone can provide me with some advice on
how to resolve this problem:

We are experiencing "holes" in tissue sections. The holes appear mainly
in the epidermis but sometimes in the dermis. I've tried a few different
things but still haven't resolved. If someone has experienced or seen
this please help. Any and all feedback will be appreciated.

I have some sample photos for your review, if anyone would like to see
what I'm talking about. Please let me know and I'll email them to you.



Thank you,

Albert











The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message._______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet