[Histonet] RE: H&E staining
Goodwin, Diana
dgoodwin <@t> rwjuhh.edu
Wed Nov 14 11:57:37 CST 2012
Jackie:
If you are using tap water to rinse, check the pH. Acidic tap water can lighten the Hematoxylin staining.
Diana Goodwin
Histology Supervisor
RWJUHH
609-631-6996
dgoodwin <@t> rwjuhh.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, November 14, 2012 11:27 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 108, Issue 18
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Today's Topics:
1. On call (Martin, Gary)
2. RE: staffing numbers (Lynette Pavelich)
3. Re: Help! Liver mistakenly processed in paraffin (had to be
in OCT instead)! (Jennifer MacDonald)
4. Re: Help! Liver mistakenly processed in paraffin (had to be
in OCT instead)! (z o n k e d)
5. Re: staffing numbers (Rene J Buesa)
6. Re: Help! Liver mistakenly processed in paraffin (had to be
in OCT instead)! (Rene J Buesa)
7. liftin tissue (Lee Loss)
8. Problem with cardiomyocytes staining (Amos Brooks)
9. Cassette and slide labelling systems (Sheila Adey)
10. RE: Re: Help! Liver mistakenly processed in paraffin (had to
be in OCT instead)! (Edwards, Richard E.)
11. Technovit 9100 new fails to polymerize (Jean-Philippe Berteau)
12. Technovit 9100 new fails to polymerize (Jean-Philippe Berteau)
13. RE: staffing numbers (Tom McNemar)
14. RE: Technovit 9100 new fails to polymerize (Marsh, Nannette)
15. TBS Slide Printer (CHRISTIE GOWAN)
16. RE: liftin tissue (Vanessa Perez)
17. Zinc formalin as primary fixative for TEM (Carla M Conway)
18. H&E Question (Jaclyn Pitts)
19. RE: H&E Question (Craven Peter (NHS HIGHLAND))
20. RE: H&E Question (Goins, Tresa)
21. Histology Supervisor Needed in Atlanta. Can you help?
(Pam Barker)
22. Re: H&E Question (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Tue, 13 Nov 2012 10:07:36 -0800
From: "Martin, Gary" <gmartin <@t> marshallmedical.org>
Subject: [Histonet] On call
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6ED9D4252F278841A0593D3D788AF24C179597D6 <@t> mailsvr.MARSHMED.local>
Content-Type: text/plain; charset="us-ascii"
This question may be a bit off subject Histonet, but I have not been
able to find information on this subject. Our facility has decided to
pay our Pathologist for their on call duties. I have been tasked with
finding what other facilities are paying for this service. Any
information would be appreciated.
Thank You
Gary
------------------------------
Message: 2
Date: Tue, 13 Nov 2012 18:54:05 +0000
From: Lynette Pavelich <LPaveli1 <@t> hurleymc.com>
Subject: [Histonet] RE: staffing numbers
To: "Hutton, Allison" <ahutton <@t> dh.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<89F4666A496DC949A819ECC40E11C867BF5692BC <@t> EXCHANGEMB1.hmc.hurleymc.com>
Content-Type: text/plain; charset="us-ascii"
We process ~14.0K cases/yr and 34.5K blocks/yr. We have 4 techs with me working part time on the bench.
Lynette
Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503
ph: 810.262.9948
mobile: 810.444.7966
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Hutton, Allison [AHutton <@t> dh.org]
Sent: Tuesday, November 13, 2012 11:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] staffing numbers
Hi Everyone,
I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs.
Any information will be appreciated
Thank you
Allison
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Tue, 13 Nov 2012 11:01:14 -0800
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin
(had to be in OCT instead)!
To: z o n k e d <zonked <@t> gmail.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>,
"histonet-bounces <@t> lists.utsouthwestern.edu"
<histonet-bounces <@t> lists.utsouthwestern.edu>
Message-ID:
<OF088521F9.9DEDEB08-ON88257AB5.00687433-88257AB5.00687BB8 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
if there was fat replacement, such as cirrhosis, you will see it in the
morphology.
From: z o n k e d <zonked <@t> gmail.com>
To: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>,
"histonet-bounces <@t> lists.utsouthwestern.edu"
<histonet-bounces <@t> lists.utsouthwestern.edu>
Date: 11/13/2012 09:43 AM
Subject: Re: Help! Liver mistakenly processed in paraffin (had to
be in OCT instead)!
We just wanted to see general lipids, nothing in particular. This mouse
died unexpectedly and may have been part of a group that was put on a high
fat or high bile acid diet and we just wanted to see what happened.
On Tuesday, November 13, 2012, Jennifer MacDonald wrote:
It depends on what you are using the oil red o for. Lipofuscin and ceroid
can be demonstrated with an oil red o stain after processing.
Jennifer MacDonald
From: z o n k e d <zonked <@t> gmail.com>
To: "histonet <@t> lists.utsouthwestern.edu" <
histonet <@t> lists.utsouthwestern.edu>
Date: 11/13/2012 08:53 AM
Subject: [Histonet] Help! Liver mistakenly processed in paraffin
(had to be in OCT instead)!
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I think I pretty much screwed up.
I should have embedded half of a mouse liver in paraffin for microtome
sectioning while the other half should have been embedded in OCT for
cryosectioning (for oil red o). I made the mistake last night of placing
both liver halves into the tissue processor. The liver I intended for OCT
embedding is now hard as wax. Is there any way to deparaffinize processed
organs and may I embed them in OCT for proper cryosectioning? I imagine
that the liver would get dehydrated, I would get crappy sections, and Oil
Red O won't work.
Any suggestions are welcome.
Thank you so much,
Zoe W.
--
"It costs nothing to say something kind. Even less to shut up altogether."
--Nathan Fillion
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
--
"It costs nothing to say something kind. Even less to shut up altogether."
--Nathan Fillion
------------------------------
Message: 4
Date: Tue, 13 Nov 2012 14:41:06 -0500
From: z o n k e d <zonked <@t> gmail.com>
Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin
(had to be in OCT instead)!
To: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>,
ryaskovich <@t> dir.nidcr.nih.gov
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>,
"histonet-bounces <@t> lists.utsouthwestern.edu"
<histonet-bounces <@t> lists.utsouthwestern.edu>
Message-ID:
<CAE7HDS6JtL=8Hw4jD0Hbdytcrf9h7k_KevNEsxyKnVdWHWU-eA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Thank you all for your helpful responses! I will go ahead and carry on with
paraffin sectioning and stain with H&E as planned. As for the Oil Red O,
I'll try that too and see what happens.
(Still amazed at how amazing Histonet is and how helpful you all are! Thank
you very much for all your responses!)
On Tue, Nov 13, 2012 at 2:01 PM, Jennifer MacDonald <JMacDonald <@t> mtsac.edu>wrote:
> if there was fat replacement, such as cirrhosis, you will see it in the
> morphology.
>
>
>
> From: z o n k e d <zonked <@t> gmail.com>
> To: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
> Cc: "histonet <@t> lists.utsouthwestern.edu" <
> histonet <@t> lists.utsouthwestern.edu>, "
> histonet-bounces <@t> lists.utsouthwestern.edu" <
> histonet-bounces <@t> lists.utsouthwestern.edu>
> Date: 11/13/2012 09:43 AM
> Subject: Re: Help! Liver mistakenly processed in paraffin (had to
> be in OCT instead)!
> ------------------------------
>
>
>
> We just wanted to see general lipids, nothing in particular. This mouse
> died unexpectedly and may have been part of a group that was put on a high
> fat or high bile acid diet and we just wanted to see what happened.
>
> On Tuesday, November 13, 2012, Jennifer MacDonald wrote:
> It depends on what you are using the oil red o for. Lipofuscin and ceroid
> can be demonstrated with an oil red o stain after processing.
> Jennifer MacDonald
>
>
>
>
> From: z o n k e d <*zonked <@t> gmail.com*>
> To: "*histonet <@t> lists.utsouthwestern.edu*" <*
> histonet <@t> lists.utsouthwestern.edu*>
> Date: 11/13/2012 08:53 AM
> Subject: [Histonet] Help! Liver mistakenly processed in paraffin
> (had to be in OCT instead)!
> Sent by: *histonet-bounces <@t> lists.utsouthwestern.edu*
> ------------------------------
>
>
>
> Hello Histonetters,
>
> First time writer, long time reader. I'm a newbie tech in academia and I
> was given a simple task which I think I pretty much screwed up.
>
> I should have embedded half of a mouse liver in paraffin for microtome
> sectioning while the other half should have been embedded in OCT for
> cryosectioning (for oil red o). I made the mistake last night of placing
> both liver halves into the tissue processor. The liver I intended for OCT
> embedding is now hard as wax. Is there any way to deparaffinize processed
> organs and may I embed them in OCT for proper cryosectioning? I imagine
> that the liver would get dehydrated, I would get crappy sections, and Oil
> Red O won't work.
>
> Any suggestions are welcome.
>
> Thank you so much,
>
> Zoe W.
>
>
> --
> "It costs nothing to say something kind. Even less to shut up altogether."
>
> --Nathan Fillion
> _______________________________________________
> Histonet mailing list*
> **Histonet <@t> lists.utsouthwestern.edu**
> **http://lists.utsouthwestern.edu/mailman/listinfo/histonet*<http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
>
>
>
> --
> "It costs nothing to say something kind. Even less to shut up altogether."
>
> --Nathan Fillion
>
>
>
--
"It costs nothing to say something kind. Even less to shut up altogether."
--Nathan Fillion
------------------------------
Message: 5
Date: Tue, 13 Nov 2012 11:42:46 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] staffing numbers
To: "Hutton, Allison" <AHutton <@t> dh.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1352835766.11849.YahooMailNeo <@t> web163102.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Allison:
The information you need is in at http://histosearch.com/rene.html
Ren? J.
________________________________
From: "Hutton, Allison" <AHutton <@t> dh.org>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, November 13, 2012 11:00 AM
Subject: [Histonet] staffing numbers
Hi Everyone,
I am looking for some crude numbers regarding staffing.? I would like to know the number of cases done per year and your number of histotechs.?
Any information will be appreciated
Thank you
Allison
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Tue, 13 Nov 2012 11:47:42 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Help! Liver mistakenly processed in paraffin
(had to be in OCT instead)!
To: z o n k e d <zonked <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1352836062.62198.YahooMailNeo <@t> web163106.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
You really screwed it up!
When you placed both pieces of liver in the processor both were subjected to the effect of ethanol and probably xylene and both reagents extracted the liver fat and no matter what you try to do now, there will be not enough fat in the pieces as to even try the ORO stain.
Try to get another piece. Anything you will try will not render good cryosections and no fat staining.
Ren? J.
________________________________
From: z o n k e d <zonked <@t> gmail.com>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, November 13, 2012 11:52 AM
Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I think I pretty much screwed up.
I should have embedded half of a mouse liver in paraffin for microtome
sectioning while the other half should have been embedded in OCT for
cryosectioning (for oil red o). I made the mistake last night of placing
both liver halves into the tissue processor. The liver I intended for OCT
embedding is now hard as wax. Is there any way to deparaffinize processed
organs and may I embed them in OCT for proper cryosectioning? I imagine
that the liver would get dehydrated, I would get crappy sections, and Oil
Red O won't work.
Any suggestions are welcome.
Thank you so much,
Zoe W.
--
"It costs nothing to say something kind. Even less to shut up altogether."
? ? --Nathan Fillion
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Tue, 13 Nov 2012 21:21:01 +0000
From: Lee Loss <LLoss <@t> dermwisconsin.com>
Subject: [Histonet] liftin tissue
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2B99CB40EC5CC7409888A2961CBF96880C32A0FC <@t> EX2010.DERM.LOCAL>
Content-Type: text/plain; charset="us-ascii"
I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the H&E and run an immuno on it. Does anyone have a procedure for doing that? Thank you.
Lee
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------------------------------
Message: 8
Date: Tue, 13 Nov 2012 17:18:51 -0500
From: Amos Brooks <amosbrooks <@t> gmail.com>
Subject: [Histonet] Problem with cardiomyocytes staining
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAC95ki-7LmqqrBfhE4XJ_Wk4nC6HYS7TxDr6c4czRrrWjLH3Tg <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Laura,
I do this stain very frequently. It can be finickey. You should make
very sure the pH is below 2.5. It could be that you have an old solution
and over time these tend to drift toward a neutral pH. This will affect the
staining. Sometimes the solutions need to be discarded and either re-made
or have new purchased.
Best of luck,
Amos
On Tue, Nov 13, 2012 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:
> Message: 18
> Date: Tue, 13 Nov 2012 18:32:09 +0100 (CET)
> From: "Laura Avogaro" <avogaro <@t> science.unitn.it>
> Subject: [Histonet] Problem with cardiomyocytes staining
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <3064.192.168.178.78.1352827929.squirrel <@t> www.science.unitn.it>
> Content-Type: text/plain;charset=iso-8859-1
>
> Dear Histonetters,
> I am new in histology so I ask if someone have experience with cardiac
> tissue. Recently I performed a Picrosirius Red staining (pig atrium, 5
> microns thickness) but in some sections I noted cardiomyocytes colored
> brown (instead of pale yellow).I wonder if it is probably due to the
> thickness of the section or something alse(unfortunately I use an old
> microtome whose probably fails to work in the correct way).
> Thank you in advance
> Best regards
>
> Laura
>
------------------------------
Message: 9
Date: Tue, 13 Nov 2012 18:14:00 -0500
From: Sheila Adey <sadey <@t> hotmail.ca>
Subject: [Histonet] Cassette and slide labelling systems
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY154-W6023F497D8F33487919D48C66C0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Hi Everyone:I'm looking for opinions on cassette and slide labelling systems. Please share your likes and dislikes.ThanksSheila
p.s Vendors Do Not call me over this email. That's very annoying
------------------------------
Message: 10
Date: Wed, 14 Nov 2012 09:00:11 +0000
From: "Edwards, Richard E." <ree3 <@t> leicester.ac.uk>
Subject: RE: [Histonet] Re: Help! Liver mistakenly processed in
paraffin (had to be in OCT instead)!
To: 'z o n k e d' <zonked <@t> gmail.com>, Jennifer MacDonald
<JMacDonald <@t> mtsac.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>,
"histonet-bounces <@t> lists.utsouthwestern.edu"
<histonet-bounces <@t> lists.utsouthwestern.edu>
Message-ID:
<7722595275A4DD4FA225B92CDBF174A101A7F49AC81A <@t> EXC-MBX3.cfs.le.ac.uk>
Content-Type: text/plain; charset="us-ascii"
Perhaps all is not lost as you will be able to see on a H <@t> E the spaces reluctantly vacated by the lipids.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of z o n k e d
Sent: 13 November 2012 17:44
To: Jennifer MacDonald
Cc: histonet <@t> lists.utsouthwestern.edu; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened.
On Tuesday, November 13, 2012, Jennifer MacDonald wrote:
> It depends on what you are using the oil red o for. Lipofuscin and
> ceroid can be demonstrated with an oil red o stain after processing.
> Jennifer MacDonald
>
>
>
>
> From: z o n k e d <zonked <@t> gmail.com <javascript:_e({}, 'cvml',
> 'zonked <@t> gmail.com');>>
> To: "histonet <@t> lists.utsouthwestern.edu <javascript:_e({}, 'cvml',
> 'histonet <@t> lists.utsouthwestern.edu');>"
> <histonet <@t> lists.utsouthwestern.edu<javascript:_e({}, 'cvml',
> 'histonet <@t> lists.utsouthwestern.edu');>
> >
> Date: 11/13/2012 08:53 AM
> Subject: [Histonet] Help! Liver mistakenly processed in paraffin
> (had to be in OCT instead)!
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu<javascript:_e({}, 'cvml', 'histonet-bounces <@t> lists.utsouthwestern.edu');>
> ------------------------------
>
>
>
> Hello Histonetters,
>
> First time writer, long time reader. I'm a newbie tech in academia and
> I was given a simple task which I think I pretty much screwed up.
>
> I should have embedded half of a mouse liver in paraffin for microtome
> sectioning while the other half should have been embedded in OCT for
> cryosectioning (for oil red o). I made the mistake last night of
> placing both liver halves into the tissue processor. The liver I
> intended for OCT embedding is now hard as wax. Is there any way to
> deparaffinize processed organs and may I embed them in OCT for proper
> cryosectioning? I imagine that the liver would get dehydrated, I would
> get crappy sections, and Oil Red O won't work.
>
> Any suggestions are welcome.
>
> Thank you so much,
>
> Zoe W.
>
>
> --
> "It costs nothing to say something kind. Even less to shut up altogether."
>
> --Nathan Fillion
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu <javascript:_e({}, 'cvml',
> 'Histonet <@t> lists.utsouthwestern.edu');>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
--
"It costs nothing to say something kind. Even less to shut up altogether."
--Nathan Fillion
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Wed, 14 Nov 2012 11:07:35 +0100
From: "Jean-Philippe Berteau" <jean-philippe.berteau <@t> tu-harburg.de>
Subject: [Histonet] Technovit 9100 new fails to polymerize
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001001cdc24f$deaa7390$9bff5ab0$@tu-harburg.de>
Content-Type: text/plain; charset="us-ascii"
Hello,
I got the same problem, how did you solve it ?
Best regards
Jean-Philippe
------------------------------
Message: 12
Date: Wed, 14 Nov 2012 11:13:58 +0100
From: "Jean-Philippe Berteau" <jean-philippe.berteau <@t> tu-harburg.de>
Subject: [Histonet] Technovit 9100 new fails to polymerize
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001d01cdc250$c32ed150$498c73f0$@tu-harburg.de>
Content-Type: text/plain; charset="us-ascii"
Hello,
As I wrote you some weeks ago, we started to use Technovit 9100 New
for tissue embedding. You helped me to solve my previous problems.
And I hope, you will help me to solve my present problems.
Sorry, I can explain my problem as follow :
The main problem is that we are having some troubles with the
polymerization of Technovit 9100 New. The polymerization mixture does
not want to become hard. We filled trial moulds with 3 ml of
polymerization mixture. Moulds were vacuumed and sealed. We tried to
perform the polymerization reaction at -30 degrees C, -20 degrees C,
-4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails
to polymerize. After a week, the polymerization mixture becomes
gelatinous but not hard. We used the manufacturer's protocol. The
preparation of the polymerization mixture was precise (I accurately prepared
stock solutions several times). I cannot understand where is the problem
May be it is necessary to add more activator (dibenzoyl peroxide and
N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions
A and B in another
proportion (not 9:1).
Is it necessary to prepare the solution at 4 degrees too ?
What should I do?
Thank you in advance,
------------------------------
Message: 13
Date: Wed, 14 Nov 2012 05:58:25 -0500
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: [Histonet] RE: staffing numbers
To: "'Hutton, Allison'" <AHutton <@t> dh.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E9A90E28259D2F4E84308C5E8EA8F7B4013865730209 <@t> lmhs-exchange>
Content-Type: text/plain; charset="us-ascii"
Allison,
We are right around 7k cases/year, 20k blocks, and about 33k slides. We have 3 tech including myself as cutter and embedder. The other two tech also cover grossing, special stains, IHC, and cytology prep.
We have 1 lab assistant who also helps out with grossing (doesn't do frozen) and cytology. We are also looking to hire another assistant to do filing, etc.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hutton, Allison
Sent: Tuesday, November 13, 2012 11:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] staffing numbers
Hi Everyone,
I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs.
Any information will be appreciated
Thank you
Allison
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------------------------------
Message: 14
Date: Wed, 14 Nov 2012 06:43:56 -0600
From: "Marsh, Nannette" <NMP <@t> stowers.org>
Subject: RE: [Histonet] Technovit 9100 new fails to polymerize
To: "'Jean-Philippe Berteau'" <jean-philippe.berteau <@t> tu-harburg.de>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2C40E43D1F7A56408C4463FD245DDDF9078ED7C148 <@t> EXCHMB-02.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
We had the same problems. We switched to Immunobed and that is wonderful. Sets up every single time. Give it a try. Best regards, Nanne
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jean-Philippe Berteau
Sent: Wednesday, November 14, 2012 4:14 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Technovit 9100 new fails to polymerize
Hello,
As I wrote you some weeks ago, we started to use Technovit 9100 New
for tissue embedding. You helped me to solve my previous problems.
And I hope, you will help me to solve my present problems.
Sorry, I can explain my problem as follow :
The main problem is that we are having some troubles with the
polymerization of Technovit 9100 New. The polymerization mixture does
not want to become hard. We filled trial moulds with 3 ml of
polymerization mixture. Moulds were vacuumed and sealed. We tried to
perform the polymerization reaction at -30 degrees C, -20 degrees C,
-4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails
to polymerize. After a week, the polymerization mixture becomes
gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem
May be it is necessary to add more activator (dibenzoyl peroxide and
N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another
proportion (not 9:1).
Is it necessary to prepare the solution at 4 degrees too ?
What should I do?
Thank you in advance,
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------------------------------
Message: 15
Date: Wed, 14 Nov 2012 15:26:26 +0000
From: CHRISTIE GOWAN <christiegowan <@t> msn.com>
Subject: [Histonet] TBS Slide Printer
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT002-W205ACEE3A3CE0BFE9F89D4CAE530 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
I am posting a question for a friend. She is looking for feedback on the TBS Shurmark slide marking instrument. All comments are appreciated. Thanks.
Christie
------------------------------
Message: 16
Date: Wed, 14 Nov 2012 09:43:00 -0600
From: Vanessa Perez <vperez <@t> pathreflab.com>
Subject: [Histonet] RE: liftin tissue
To: Lee Loss <LLoss <@t> dermwisconsin.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C80B5EE351AAD74DADAB70FABCB78D193BCDEDEFA0 <@t> exchange.pasa.local>
Content-Type: text/plain; charset="us-ascii"
We use Mount quick media, which comes with a procedure for section transfer....
Vanessa
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee Loss
Sent: Tuesday, November 13, 2012 3:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] liftin tissue
I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the H&E and run an immuno on it. Does anyone have a procedure for doing that? Thank you.
Lee
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------------------------------
Message: 17
Date: Wed, 14 Nov 2012 07:43:56 -0800
From: Carla M Conway <cmconway <@t> usgs.gov>
Subject: [Histonet] Zinc formalin as primary fixative for TEM
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF5B1D51F6.85CC419C-ON88257AB6.0055FF46-88257AB6.00566BB1 <@t> usgs.gov>
Content-Type: text/plain; charset="US-ASCII"
Hello everyone,
A colleague has tissues which are fixed in zinc formalin and wants to
examine them by transmission electron microscopy. Any thoughts regarding
the use of this fixative for EM?
Thanks in advance,
Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmconway <@t> usgs.gov
------------------------------
Message: 18
Date: Wed, 14 Nov 2012 09:50:34 -0600
From: Jaclyn Pitts <pitts.jaclyn <@t> gmail.com>
Subject: [Histonet] H&E Question
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CANpxt2kLJR3Minsh55KDXBGkyb65byXVaumhB8WcPC0o-tMuOw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi All,
I am having a problem with my staining, ( well, I think its pretty good but
the path says its not cark enough for her)
So, I am using Azer Scientific Hematoxylin Extra and I initially started
out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The
problem is the control I have works and looks great at the 2.5 min. Besides
just keeping it in the heme longer what else can I do? I have cut a few
extra dummy slides today so I can try different things out with them. I am
tired of hearing from the path everyday that the heme is too light for her.
I am a new lab for a derm clinic and I work alone so its basiclly up to me
to figure this out. Please help! Thanks!
Jackie
------------------------------
Message: 19
Date: Wed, 14 Nov 2012 15:52:11 +0000
From: "Craven Peter (NHS HIGHLAND)" <peter.craven <@t> nhs.net>
Subject: RE: [Histonet] H&E Question
To: Jaclyn Pitts <pitts.jaclyn <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20121114155324.DDD98448D6F <@t> nhs-pd1e-esg101.ad1.nhs.net>
Content-Type: text/plain; charset="us-ascii"
Jaclyn
it sounds daft but check the pH of your Eosin we found this can be strongly acidic.
Peter
Peter L Craven FIBMS
Pathology Department
Raigmore Hospital
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts [pitts.jaclyn <@t> gmail.com]
Sent: 14 November 2012 03:50 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E Question
Hi All,
I am having a problem with my staining, ( well, I think its pretty good but
the path says its not cark enough for her)
So, I am using Azer Scientific Hematoxylin Extra and I initially started
out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The
problem is the control I have works and looks great at the 2.5 min. Besides
just keeping it in the heme longer what else can I do? I have cut a few
extra dummy slides today so I can try different things out with them. I am
tired of hearing from the path everyday that the heme is too light for her.
I am a new lab for a derm clinic and I work alone so its basiclly up to me
to figure this out. Please help! Thanks!
Jackie
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------------------------------
Message: 20
Date: Wed, 14 Nov 2012 16:02:57 +0000
From: "Goins, Tresa" <TGoins <@t> mt.gov>
Subject: RE: [Histonet] H&E Question
To: Jaclyn Pitts <pitts.jaclyn <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CA4DF32ED505D94BB55E95487D8E98413085494B <@t> DOAISD5205.state.mt.ads>
Content-Type: text/plain; charset="us-ascii"
Jackie -
Try a different hematoxylin - we switched to Platinum Line Modified Harris Hematoxylin - we save money and the pathologists prefer the results.
Tresa
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts
Sent: Wednesday, November 14, 2012 8:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E Question
Hi All,
I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5 min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her.
I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks!
Jackie
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Wed, 14 Nov 2012 11:11:34 -0500
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] Histology Supervisor Needed in Atlanta. Can you
help?
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <046c01cdc282$b7f69520$27e3bf60$@earthlink.net>
Content-Type: text/plain; charset="us-ascii"
Hi Histonetters!
I hope everybody is having a great day. I wanted to drop you a quick line
to ask for help because I am currently recruiting for one of my best
clients located in Atlanta, GA. We are looking for an experienced Histology
Supervisor. We are looking for someone who is ASCP certified with at least
3 years of histology and 2 years of experience supervising a histology lab.
They are offering a great salary, terrific benefits, the stability of a
large company and a great group of people to work with.
The help I need from you is do you know anyone that might be interested in
hearing about this opportunity? If so could you please forward my e-mail to
them?
If you are interested in this position please contact me ASAP at
relia1 <@t> earthlink.net or toll free at 866-607-3542.
Remember if I hire someone you refer you will receive a referral bonus!
Thank you,
Pam - 866-607-3542 (866-60RELIA) - Toll Free
Thank You!
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: relia1 <@t> earthlink.net
www.facebook.com <http://www.facebook.com/PamBarkerRELIA> /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia
------------------------------
Message: 22
Date: Wed, 14 Nov 2012 08:20:00 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] H&E Question
To: Jaclyn Pitts <pitts.jaclyn <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1352910000.32673.YahooMailNeo <@t> web163101.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I never heard of "Azer Scientific hematoxylin" but if you can leave the sections in it during 12 minutes and it is still "weak" for your pathologist, it seems that it is a "progressive" hematoxylin, of the Mayer type.
Progressive hematoxylins are somewhat tricky and have to be very fresh to work well.
I have always used and preferred "regressive" hematoxylins of the Harris' type. With them you are in control of the staining when you differentiate them up to the strength?proffered by your pathologist.
My advise, since it seems that you cannot satisfy your pathologist's demands,is to switch to a Harris type hematoxylin and stop fiddling with the hematoxylin you are using. I always used and preferred Harris by Richard Alan. Switch and your problems will be over.
Ren? J.
________________________________
From: Jaclyn Pitts <pitts.jaclyn <@t> gmail.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, November 14, 2012 10:50 AM
Subject: [Histonet] H&E Question
Hi All,
I am having a problem with my staining, ( well, I think its pretty good but
the path says its not cark enough for her)
So, I am using Azer Scientific Hematoxylin Extra and I initially started
out with 2.5 min in it. I upped it to 5? min and then to 10 and 12 min. The
problem is the control I have works and looks great at the 2.5 min. Besides
just keeping it in the heme longer what else can I do? I have cut a few
extra dummy slides today so I can try different things out with them. I am
tired of hearing from the path everyday that the heme is too light for her.
I am a new lab for a derm clinic and I work alone so its basiclly up to me
to figure this out. Please help! Thanks!
Jackie
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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End of Histonet Digest, Vol 108, Issue 18
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