[Histonet] Problem with cardiomyocytes staining

Laura Avogaro avogaro <@t> science.unitn.it
Tue Nov 13 11:32:09 CST 2012

Dear Histonetters,
I am new in histology so I ask if someone have experience with cardiac
tissue. Recently I performed a Picrosirius Red staining (pig atrium, 5
microns thickness) but in some sections I noted cardiomyocytes colored
brown (instead of pale yellow).I wonder if it is probably due to the
thickness of the section or something alse(unfortunately I use an old
microtome whose probably fails to work in the correct way).
Thank you in advance
Best regards


Laura Avogaro
Center for Biomedical Technologies (BIOTECH)
University of Trento

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