[Histonet] radiation damage to nerves

[Rene J Buesa]

I think that is a good approach but I do not think that an inclusion in GMA plastic is required.
A well infiltrated FFPE tissue can be sectioned at 1-2 µm
René J.



________________________________
From: Patsy Ruegg <pruegg <@t> ihctech.net>
To: 'Histonet' <histonet <@t> lists.utsouthwestern.edu> 
Sent: Monday, November 12, 2012 4:16 PM
Subject: [Histonet] radiation damage to nerves 

Does anyone have any ideas on how we might demonstrate radiation injury to
peripheral nerves near the prostate (in a canine model).  we would like to
evaluate demyelinating changes and it has been suggested we might do this by
embedding the tissue in GMA plastic and cutting 2 micron sections and doing
toludine blue stain?????  We are trying to avoid osmium and EM for this.
Any ideas?



Regards,



Patsy



Patsy Ruegg, HT(ASCP)QIHC

Ruegg IHC Consulting, LLC

40864 Arkansas Ave

Bennett, CO 80102

Phone: 303-644-4538

Fax: 720-859-4110

<mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net



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