[Histonet] Stain for osteoporotic bone in Spurr plastic

[Malandra, Allison E]

Hi there -

  We are trying to stain osteoporotic bone that was embedded using Spurr plastic.  We need to be able to differentiate osteoid from mineralized bone.  We have tried Gio's trichrome and Goldner's trichrome with no success, both surface staining and on deplasticized slides.  We are thinking of trying Von Kossa, does anyone have a recipe/protocol for this?  Or other types of stains that you have had success with?  Thank you!

Allison Malandra, DVM
University of Iowa, Bone Healing Research Lab


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