AW: [Histonet] Re: [Histology] Congo Red Stain

[Rene J Buesa]

For a strong staining with Congo Red and nuclear staining with hematoxylin, do the hematoxylin first of all steps and later stain for Congo Red.
Doing the hematoxylin last will produce weak nuclear staining.
René J.


________________________________
From: Gudrun Lang <gu.lang <@t> gmx.at>
To: "'Sherwood, Margaret'" <MSHERWOOD <@t> PARTNERS.ORG> 
Cc: Histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, November 8, 2012 11:32 AM
Subject: AW: [Histonet] Re: [Histology] Congo Red Stain

We use the Merck kit but have turned the order of reagenses: first congored
and differentiation, then hemalaun. That masks much of unwanted staining.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Sherwood,
Margaret
Gesendet: Donnerstag, 08. November 2012 16:01
An: 'histonet <@t> lists.utsouthwestern.edu'
Betreff: [Histonet] Re: [Histology] Congo Red Stain

To all:

Would people please share their method for doing Congo Red staining?  (We
are not interested in making up the reagents from powder form).

Thanks!
Peggy


Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org



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