[Histonet] RE: Protocol for mouse tissue on VIP Processor

[Elizabeth Chlipala]

We use that processor for soft tissue we use 20 minutes per station for kidney and lung and liver - trimmed sections, whole or half kidneys will require more time anywhere from 30 to 45 minutes per station. For Brain 3 mm section we use 30 minutes per station and for fat I would go 45 minutes to 1 hour per station.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of D'Andreas Williams
Sent: Wednesday, November 07, 2012 3:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Protocol for mouse tissue on VIP Processor

To All histology experts:



We are currently using a VIP 6 Processor. We have a high demand for
mouse tissue. Does anyone out there have a protocol for mouse brain (3mm
sections), liver, kidney (whole or half), heart, lung, and fat? If you
could share your protocol as well as vac and pressure settings we would
be grateful.



With Kindest Regards,



D'Andreas Williams

Pennington Biomedical Research Center

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