[Histonet] Re: Histonet Digest, Vol 108, Issue 8

Madeleine Huey madeleinehuey <@t> gmail.com
Tue Nov 6 14:22:51 CST 2012


Leica Cryostat CM1850 UV for me!  Used them for more then 20 years and
still prefer them.:)
Madeleine Huey

On Tue, Nov 6, 2012 at 10:00 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
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> Today's Topics:
>
>    1. Looking at cryostats (kgrobert <@t> rci.rutgers.edu)
>    2. Subject: [Histonet] Background staining on H pylori (Tim Higgins)
>    3. Re: Looking at cryostats (Rene J Buesa)
>    4. RE: Looking at cryostats (Marcum, Pamela A)
>    5. Re: cost per H&E (Rene J Buesa)
>    6. Fw: [Histonet] Cytology in Jasper, Indiana
>       (Sara Baldwin/mhhcc.org)
>    7. RE: Looking at cryostats (Bea DeBrosse-Serra)
>    8. RE: Looking at cryostats (Morken, Timothy)
>    9. RE: Looking at cryostats (Horn, Hazel V)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 6 Nov 2012 09:25:45 -0500 (EST)
> From: kgrobert <@t> rci.rutgers.edu
> Subject: [Histonet] Looking at cryostats
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>         <2ccd020a326d6f64ee276b51bdbcb637.squirrel <@t> webmail.rci.rutgers.edu>
> Content-Type: text/plain;charset=iso-8859-1
>
> What's good out there these days?  I am currently looking at the Leica
> CM3050; are there other brands that I should consider?
>
> Thanks so much,
> Kathleen
>
>
> Principal Lab Technician
> Neurotoxicology Labs
> Molecular Pathology Facility Core
> Dept of Pharmacology & Toxicology
> Rutgers, the State University of NJ
> 41 B Gordon Road
> Piscataway, NJ 08854
> (848) 445-1443
> FAX (732) 445-6905
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 6 Nov 2012 08:27:10 -0600
> From: "Tim Higgins" <thiggins <@t> cddmedical.com>
> Subject: Subject: [Histonet] Background staining on H pylori
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <1FC29FDEC8AD4192877047691E0725F0 <@t> cdd.loc>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
>         reply-type=original
>
> Hey Deloris,
>
> I would have the dispensing nozzles on the XT checked, could be it is not
> getting rinsed properly.  Had that issue with my Ultra not to long ago.  No
> warnings or errors showed up, just a clogged nozzle tip.
>
> Tim
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Deloris
> Carter
> Sent: Monday, November 05, 2012 5:16 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Background staining on H pylori
>
> Hi,
> I'm getting a lot of background staining on my HP's.  We use a Ventana
> BenchmarkXT with ultraView DAB.  The problem seems to be escalating of late,
> and I'm not sure why.  We use Hollande's on our GI biopsies, and run them on
> a shorter run.  Nothing has changed in the processing of the specimens, or
> the IHC procedure.  The antibody dispenser is a newer lot, but not brand
> new, as we get the larger size due to the high volume of HP's we run. Any
> ideas?
>
> Deloris Carter HT(ASCP)
> _______________________________________________
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>
>
> ------------------------------
>
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> End of Histonet Digest, Vol 108, Issue 7
> ****************************************
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 6 Nov 2012 06:34:21 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Looking at cryostats
> To: "kgrobert <@t> rci.rutgers.edu" <kgrobert <@t> rci.rutgers.edu>,      histonet
>         <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>         <1352212461.21616.YahooMailNeo <@t> web163104.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Not really! For me the Leica is the best.
> René J.
>
>
> ________________________________
> From: "kgrobert <@t> rci.rutgers.edu" <kgrobert <@t> rci.rutgers.edu>
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Sent: Tuesday, November 6, 2012 9:25 AM
> Subject: [Histonet] Looking at cryostats
>
> What's good out there these days?  I am currently looking at the Leica
> CM3050; are there other brands that I should consider?
>
> Thanks so much,
> Kathleen
>
>
> Principal Lab Technician
> Neurotoxicology Labs
> Molecular Pathology Facility Core
> Dept of Pharmacology & Toxicology
> Rutgers, the State University of NJ
> 41 B Gordon Road
> Piscataway, NJ 08854
> (848) 445-1443
> FAX (732) 445-6905
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 4
> Date: Tue, 6 Nov 2012 14:35:21 +0000
> From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
> Subject: RE: [Histonet] Looking at cryostats
> To: "'kgrobert <@t> rci.rutgers.edu'" <kgrobert <@t> rci.rutgers.edu>, histonet
>         <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>         <41D3A1AF6FEF0643BDC89E0516A6EA328A55BB26 <@t> Mail2Node1.ad.uams.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> The CM3050s is the best in my opinion for research or heavy duty frozen sectioning and it can work with the Cryo Jane system or similar if needed.
>
> Pam Marcum
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kgrobert <@t> rci.rutgers.edu
> Sent: Tuesday, November 06, 2012 8:26 AM
> To: histonet
> Subject: [Histonet] Looking at cryostats
>
> What's good out there these days?  I am currently looking at the Leica CM3050; are there other brands that I should consider?
>
> Thanks so much,
> Kathleen
>
>
> Principal Lab Technician
> Neurotoxicology Labs
> Molecular Pathology Facility Core
> Dept of Pharmacology & Toxicology
> Rutgers, the State University of NJ
> 41 B Gordon Road
> Piscataway, NJ 08854
> (848) 445-1443
> FAX (732) 445-6905
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
> ------------------------------
>
> Message: 5
> Date: Tue, 6 Nov 2012 06:37:09 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] cost per H&E
> To: Cheryl <tkngflght <@t> yahoo.com>,       "histonet <@t> lists.utsouthwestern.edu"
>         <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>         <1352212629.10960.YahooMailNeo <@t> web163102.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> That is quite acceptable cost.
> Under separate cover I am sending my costs.
> René J.
>
>
> ________________________________
> From: Cheryl <tkngflght <@t> yahoo.com>
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Monday, November 5, 2012 10:02 PM
> Subject: [Histonet] cost per H&E
>
>
>
> Depending on how I dice up labor and materials, I come up with a cost per slide (H&E), from receipt of specimen to the slide in the Path's hands ranging everywhere from $6 to $9/ slide.
>
> HELP?  How do you distill your cost per slide??
>
> Thanks,
>
> Cheryl
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
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>
> ------------------------------
>
> Message: 6
> Date: Tue, 6 Nov 2012 10:19:56 -0500
> From: "Sara Baldwin/mhhcc.org" <sbaldwin <@t> mhhcc.org>
> Subject: Fw: [Histonet] Cytology in Jasper, Indiana
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>         <OF5ACA6C19.05F87E19-ON85257AAE.00543948-85257AAE.0054394E <@t> mhhcc.org>
> Content-Type: text/plain;       charset=ISO-8859-1
>
> Good Morning Histonetters,
>
> We are currently looking for a registered Cytotechnologist to work in a Regional hospital doing all aspects of cytology.  The position is full time requiring someone with a clinical Cytology background with processing, screening, assisting on FNA's and learning Cervista HPV HR and Probetec for GC/CT.  This is a challenging position with all aspects of cytology and molecular as well.
>
>
> Please contact me directly by e-mail or check mhhcc.org website under careers to apply through the system.
>
> Thank You,
>
>
> Thanks
> Histology/Cytology Supervisor
> S. Kathy Baldwin, SCT (ASCP)
> sbaldwin <@t> mhhcc.org
> Memorial Hospital and Health Care Center
> 800 West 9th Str.
> Jasper, In 47546
> Ph 812-996-0210, 0216, Fax 812-996-0232,
> Pager 812-481-0897
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 7
> Date: Tue, 6 Nov 2012 07:37:45 -0800
> From: Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com>
> Subject: RE: [Histonet] Looking at cryostats
> To: "'kgrobert <@t> rci.rutgers.edu'" <kgrobert <@t> rci.rutgers.edu>, histonet
>         <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>         <493CAA64F203E14E8823737B9EE0E25F092CE34CE5 <@t> EXCHMB01.isis.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Leica!!!!
>
> Beatrice DeBrosse-Serra HT(ASCP)QIHC
> Isis Pharmaceuticals
> Antisense Drug Discovery
> 2855 Gazelle Ct.
> Carlsbad, CA 92010
> 760-603-2371
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kgrobert <@t> rci.rutgers.edu
> Sent: Tuesday, November 06, 2012 6:26 AM
> To: histonet
> Subject: [Histonet] Looking at cryostats
>
> What's good out there these days?  I am currently looking at the Leica CM3050; are there other brands that I should consider?
>
> Thanks so much,
> Kathleen
>
>
> Principal Lab Technician
> Neurotoxicology Labs
> Molecular Pathology Facility Core
> Dept of Pharmacology & Toxicology
> Rutgers, the State University of NJ
> 41 B Gordon Road
> Piscataway, NJ 08854
> (848) 445-1443
> FAX (732) 445-6905
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 6 Nov 2012 16:08:25 +0000
> From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
> Subject: RE: [Histonet] Looking at cryostats
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <761E2B5697F795489C8710BCC72141FF02B6F8 <@t> ex07.net.ucsf.edu>
> Content-Type: text/plain; charset=us-ascii
>
> Thermo NX70 is very nice. In fact everyone fights to use it rather than the old CM3050 we have...
>
> We use them for frozen sections for kidney, skin IF and muscle histochemistry. Lots and lots of frozens...
>
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kgrobert <@t> rci.rutgers.edu
> Sent: Tuesday, November 06, 2012 6:26 AM
> To: histonet
> Subject: [Histonet] Looking at cryostats
>
> What's good out there these days?  I am currently looking at the Leica CM3050; are there other brands that I should consider?
>
> Thanks so much,
> Kathleen
>
>
> Principal Lab Technician
> Neurotoxicology Labs
> Molecular Pathology Facility Core
> Dept of Pharmacology & Toxicology
> Rutgers, the State University of NJ
> 41 B Gordon Road
> Piscataway, NJ 08854
> (848) 445-1443
> FAX (732) 445-6905
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 6 Nov 2012 10:13:54 -0600
> From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
> Subject: RE: [Histonet] Looking at cryostats
> To: "'kgrobert <@t> rci.rutgers.edu'" <kgrobert <@t> rci.rutgers.edu>, histonet
>         <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>         <25A4DE08332B19499904459F00AAACB719BE1353F3 <@t> EVS1.archildrens.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Leica!
>
> Hazel Horn
> Supervisor of Histology/Autopsy/Transcription
> Anatomic Pathology
> Arkansas Children's Hospital
> 1 Children's Way | Slot 820| Little Rock, AR 72202
> 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
> hornhv <@t> archildrens.org
> archildrens.org
>
>
>
>
> 100 YEARS YOUNG!
> JOIN THE PARTY AT
> ach100.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kgrobert <@t> rci.rutgers.edu
> Sent: Tuesday, November 06, 2012 8:26 AM
> To: histonet
> Subject: [Histonet] Looking at cryostats
>
> What's good out there these days?  I am currently looking at the Leica CM3050; are there other brands that I should consider?
>
> Thanks so much,
> Kathleen
>
>
> Principal Lab Technician
> Neurotoxicology Labs
> Molecular Pathology Facility Core
> Dept of Pharmacology & Toxicology
> Rutgers, the State University of NJ
> 41 B Gordon Road
> Piscataway, NJ 08854
> (848) 445-1443
> FAX (732) 445-6905
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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> End of Histonet Digest, Vol 108, Issue 8
> ****************************************



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