[Histonet] RNA isolation form stained slides

[Mark Tarango]

Have you tried using more sections during extraction?  Can you extract into
a smaller volume?

On Friday, November 2, 2012, Vanessa Orsini wrote:

>
>
>
>
>
>
> Hello,
>
> I need to extract RNA for a RT-PCR after Laser Micro
> Dissection on xGal stained slides.
>
> I tried using sections from unfixed frozen organs. I fixed the
> sections in EtOH70% for 10min and then I stained them with xGal for 3h at
> 37°C.
> After air drying I cut out with the LCM and extract RNA with the PicoPure
> kit
> from Applied Biosystem. So far I didn’t manage to get enough RNA.
>
> I tried to add RNase inhibitors to all the solutions but it
> didn’t help.
>
>
>
> Any idea/suggestion?
>
> Do someone think it would be better to do a LacZ antibody staining
> on FFPE sections and extract RNA with an appropriate kit? The RNase would
> they
> be less active?
>
>
>
> Thank in advance for any help you can give me J Vanessa
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu <javascript:;>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>