[Histonet] Embedding

[Stephenson, Sheryl]

That was my initial thought,  check your paraffin.  I could be coming from the paraffin...... or whatever you are cleaning with make sure its dust free/debris free.

Sheryl Stephenson | Histology Technician 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amos Brooks
Sent: Sunday, May 27, 2012 11:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Embedding

Hi,
     Any chance it is from your reservoir? Sometimes the paraffin is not
filtered very well and there is some junk that collects in the chamber.
(Yes I'm looking at you Paraplast!) Also is the reservoir you keep your
cassettes in prior to embedding clean? Perhaps junk on your tampers?

Amos


On Sat, May 26, 2012 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 1
> Date: Fri, 25 May 2012 13:02:29 -0400 (EDT)
> From: Ann Specian <thisisann <@t> aol.com>
> Subject: [Histonet] Embedding
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <8CF08AF4CF811BC-C74-9030 <@t> webmail-m025.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
> We are having a problem with floaters in our blocks which occur during
> embedding.  We have multiple forceps which are placed in heated wells and
> each cassette is embedded with a new forcep.  We also wipe with a gauze,
> but we are still getting floaters embedded in the cassette from time to
> time.
>
> Does anyone do anything else to prevent this?
> Thank you, Ann
>
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