[Histonet] IHC for p21, p27, and RB

Fri May 25 14:24:44 CDT 2012

Calbiochem p21 (1:100) and Cell Signaling for the p27 (1:250)
We use the Dako Flex Reagents on the autostainer.   Low pH retrieval.
I have more detailed protocol if you need it. 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun <@t> harthosp.org]
Sent: Friday, May 25, 2012 1:24 PM
To: Histonet
Subject: [Histonet] IHC for p21, p27, and RB

What has been people's experience with immunohistochemical staining for p21, p27, and Retinoblastoma (RB) gene protein on formalin-fixed, paraffin-embedded human tissue?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet