[Histonet] Neutrophil Antibodies

[John Shelley]

Hi All in Histoland,

Problem solving never ends! I am working up two antibodies for neutrophils which are NIMP-R14 from Abcam which it is a rat monoclonal and also Anti-Neutrophil Elastase also from Abcam but this is a Rb polyclonal. I have tried different conditions without any luck and was wondering if anyone has worked with these antibodies either from Abcam or another supplier and might have working conditions that have given results in the past. We are trying this on FFPE sections which the antibodies say they work on but I am thinking that I may try frozens. I was trying to avoid this however because I am using these antibodies on mouse WAT. Any help would be appreciated. Thanks!!!

Happy Monday morning!!!
Kind Regards!
John J Shelley