Subject: [Histonet] .._Bone Marrows

Thu May 3 12:21:14 CDT 2012

 Hi Valerie,
We fix bone marrow cores in B-Plus for a minimum of 2 hours, rinse then decal in Rapid Cal Immuno (with agitation) for 30 minutes. Both chemicals are supplied by BBC Biochemical (1-800-635-4477)
Babiker Bedri
MGH Pathology
Boston, MA 02114
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, May 03, 2012 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 102, Issue 3

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Today's Topics:

   1. RE: Re: Frozen brains on cryostat  (Thurby, Christina)
   2. Grossing chair (jsjurczak <@t> comcast.net)
   3. RE: DeCloaking Chamber (Cynthia Pyse)
   4. IMF antibody validation (Sebree Linda A)
   5. DeCloaking Chamber (Amos Brooks)
   6. Phosphatase Treatment (Courtney Pierce)
   7. RE: Frozen brains on cryostat (Reynolds,Donna M)
   8. .._Bone Marrows (Hannen, Valerie)
   9. Histology jobs (K.C. Carpenter)
  10. Re: .._Bone Marrows (Rene J Buesa)
  11. (no subject) (Brendal Finlay)

----------------------------------------------------------------------

Message: 1
Date: Wed, 2 May 2012 13:42:00 -0400
From: "Thurby, Christina" <christina.thurby <@t> bms.com>
Subject: [Histonet] RE: Re: Frozen brains on cryostat 
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<E9C77491626EBF41BD449EA1098B04060624AB13DA <@t> ushpwbmsmmp007.one.ads.bms.com>

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Andrea,
A couple of suggestions for you to help with sectioning the brain tissue.  If you haven't already adjusted the cryostat temperature that is a place to start.  Brains section really well at -10 to -15 C.  Make sure to let the blocks equilibrate to the temperature in the cryostat.  If you've taken the frozen blocks out of a -70 C freezer, let them sit in the cryostat for at  least 20-30 minutes to 'warm up' to the cryostat temperature.  At times, I have really good luck getting nice sections when I do the following:
1.  Shave (or face off) the block until you're ready to get a section.
2.  Using a gloved hand, place your thumb over the tissue for a few seconds.
3.  Begin sectioning - discard the first section that comes off, the second section is usually really nice (be ready to use your chilled brush) and gently pull the section toward you as you are moving the wheel (manual instrument).
4.  This method can be repeated several times - just be patient.  It does take some practice.

Finally, if you have any trouble getting the brain sections to stay adhered to the glass slides, depending on the downstream application you may want to try using the Gold Plus Slides (I think they are from Thermo Shandon or maybe Erie Scientific??)  These slides are really nice to use when working with brain tissue if you experience 'tissue lifting' during staining applications - but in our experience they can't be used for LCM work.
Good Luck!
Kristie

Christina Thurby
Bristol Myers Squibb
812-307-2093

>> On Tue, May 1, 2012 at 12:53 PM, Andrea Ferullo (non-Celgene) <
>> aferullo <@t> celgene.com> wrote:
>>
>>> Hello everyone,
>>>
>>> I recently received rat brain samples that were frozen in liquid nitrogen
>>> and embedded in OCT.  I sectioned them on the cryostat and they are coming
>>> out very wrinkled, no matter what technique I use to pick them up.  I would
>>> appreciate any tips/tricks  that anyone has to offer.  Forebrain sections
>>> are ok, but mid, hind-brain, and cerebellum are giving me a very hard time.
>>> Thanks and I look forward to your advice.
>>>
>>> Andrea

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------------------------------

Message: 2
Date: Wed, 2 May 2012 17:48:40 +0000 (UTC)
From: jsjurczak <@t> comcast.net
Subject: [Histonet] Grossing chair
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<829751199.2026664.1335980920084.JavaMail.root <@t> sz0094a.emeryville.ca.mail.comcast.net>

Content-Type: text/plain; charset=utf-8

Does anybody out there have a grossing chair that they could recommend to us? We're shopping for something that's more amenable to 4 or 5 hour sessions. We're sitting at a Mopec grossing station. Thanks 

------------------------------

Message: 3
Date: Wed, 2 May 2012 15:10:18 -0400
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: RE: [Histonet] DeCloaking Chamber
To: "'Heckford, Karen - SMMC-SF'" <Karen.Heckford <@t> DignityHealth.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003901cd2897$3721b0e0$a56512a0$@com>
Content-Type: text/plain;	charset="us-ascii"

Karen
I would just purchase a timer to plug the Decloaking chamber into, I use one
to heat my water bath for pretreatments prior to by techs coming in. I
purchased the timers from Fisher, cat. #666224 for $28.82. Works like a
charm. 
Cindy

Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
716-250-9235 etx. 232
e-mail cpyse <@t> x-celllab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Heckford,
Karen - SMMC-SF
Sent: Wednesday, May 02, 2012 8:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] DeCloaking Chamber

Hi,
Does anyone know of a delayed start Decloaking chamber that is independent
from the IHC stainer?  I am looking around to get a new Decloaking chamber.
Any suggetions?

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford <@t> dignityhealth.org

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------------------------------

Message: 4
Date: Wed, 2 May 2012 15:02:36 -0500
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: [Histonet] IMF antibody validation
To: <Histonet <@t> lists.utsouthwestern.edu>
Cc: Haack Lori A <LHaack <@t> uwhealth.org>
Message-ID:
	<F0B9FD4B28C80E43996FFD4408B198AE01EFF3F5 <@t> UWHC-MAIL3.uwhis.hosp.wisc.edu>

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Hello everyone,

I'm branching out into IMF antibodies and as such am starting to
validate these antibodies.  Previously no comparison studies were done
that I know of.  Now we're running frozen tissue slides manually with
our current set of antibodies simultaneously with new vendors'
antibodies being run automated.  Since IMF is not permanent, my question
to all of you is, are you photographing the slides for a permanent
record or just keeping a written record of the comparison results?

Any and all responses are welcome.

Thanks,
Linda Sebree

------------------------------

Message: 5
Date: Wed, 2 May 2012 16:46:08 -0400
From: Amos Brooks <amosbrooks <@t> gmail.com>
Subject: [Histonet] DeCloaking Chamber
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<CAC95ki93Rncjx=4mh8AK7A48hY2Jk8mGTZBE7MK_9E7sfmkPAw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi,
     Here are the best steamers on the market...
http://www.walmart.com/ip/Black-Decker-7-Quart-Food-Steamer/14320967?findingMethod=rr

     There are others that will work as well. Use only one layer. The
second layer is never as hot as the first. I drill a hole in the top and
drop in a thermometer to monitor the temperature in real time. If you spend
over $50 on such a product you are getting robbed.

Happy shopping,
Amos

On Wed, May 2, 2012 at 1:01 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 10
> Date: Wed, 2 May 2012 05:21:56 -0700
> From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> DignityHealth.org>
> Subject: [Histonet] DeCloaking Chamber
> To: "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <3328693C43A557458850CC37CE16CD1877954A2D <@t> chw-msg-829.chw.edu>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Hi,
> Does anyone know of a delayed start Decloaking chamber that is independent
> from the IHC stainer?  I am looking around to get a new Decloaking chamber.
>   Any suggetions?
>
> Karen Heckford HT ASCP CE
>

------------------------------

Message: 6
Date: Thu, 3 May 2012 10:13:04 -0400
From: Courtney Pierce <Courtney.Pierce <@t> quintiles.com>
Subject: [Histonet] Phosphatase Treatment
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<E596AA0B8A45794A8EF828F5158C44E24B32F4046F <@t> USADC-AMBXD00.quintiles.net>

Content-Type: text/plain; charset="us-ascii"

Does anyone have any info about Phosphatase Treatment and how to use it?

Thanks
Courtney Pierce
IHC Specialist
Quintiles
Translational R&D - Oncology
Innovation
Navigating the new health

610 Oakmont Lane
Westmont, IL 60559

Office: + 630-203-6234
courtney.pierce <@t> quintiles.com

clinical | commercial | consulting | capital

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Message: 7
Date: Thu, 3 May 2012 09:42:27 -0500
From: "Reynolds,Donna M" <dreynold <@t> mdanderson.org>
Subject: [Histonet] RE: Frozen brains on cryostat
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<785BBF0C5F49CE41BA74460A43A08F02315AC3F02F <@t> DCPWVMBXC0VS3.mdanderson.edu>

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I cut mouse brains frozen in OCT with very little problem.  I bring the Cyrostat temp up to between -15 to -13. Pick them up with a warm slide and seldom have a wrinkle. Brains are  unfixed placed in OCT and frozen with liquid nitrogen.
Donna Reynolds, Chief Histology Lab (IHC)
U.T,  M.D. Anderson Cancer Center
Houston, Texas
713-792-8106

Message: 11
Date: Tue, 1 May 2012 12:53:13 -0400
From: "Andrea Ferullo (non-Celgene)" <aferullo <@t> celgene.com>
Subject: [Histonet] Frozen brains on cryostat
To: "Histonet <@t> lists.utsouthwestern.edu"
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<24701B0EF4127F4FA262751AA9674EEA029FE97ED479 <@t> SUMEXPRDMB03.celgene.com>

Content-Type: text/plain; charset="us-ascii"

Hello everyone,

I recently received rat brain samples that were frozen in liquid nitrogen and embedded in OCT.  I sectioned them on the cryostat and they are coming out very wrinkled, no matter what technique I use to pick them up.  I would appreciate any tips/tricks  that anyone has to offer.  Forebrain sections are ok, but mid, hind-brain, and cerebellum are giving me a very hard time.  Thanks and I look forward to your advice.

Andrea

************

------------------------------

Message: 8
Date: Thu, 3 May 2012 11:26:29 -0400
From: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>
Subject: [Histonet] .._Bone Marrows
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <450B7A81EDA0C54E97C53D60F00776C322E7D40DF7 <@t> isexstore03>
Content-Type: text/plain; charset="us-ascii"

Good morning Histonetters!!!

Our Pathologist has been reading again !!  He read an article about Bone Marrow fixation. I have a few questions to pose to you all.

1) Do you typically fix your Bone Marrow biopsies overnight before decalcification?  We currently do not.

2) What are you fixing them in?

 Formalin?        Zinc Formalin? (this is what we currently are using)       B Plus? (this was the fixative mentioned in the article).

Any and all input will be greatly appreciated!!

Valerie

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com

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Message: 9
Date: 3 May 2012 11:28:35 -0400
From: "K.C. Carpenter" <kc <@t> ka-recruiting.com>
Subject: [Histonet] Histology jobs
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <212107475.1336058915414.JavaMail.cfservice <@t> sl4app2>
Content-Type: text/plain; charset="utf-8"

Dear Histonet Community,

I hope you are all having a great week. I am a one of the founders of a nationally recognized healthcare recruiting firm. I help Lab Professionals find permanent employment and I wanted to see if you are interested in taking the next step in your career. We are completely free of charge to candidates and and we work on laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great positions that you may be interested in including a Histotech position with a hospital outside of Columbus, Ohio.

This is a 1st shift Histotechnologist position at one of Ohio's most highly regarded hospitals. Recognized for superior patient satisfaction this 300 bed, level 1 trauma center hospital offers an excellent work environment. This is a position offers a terrific compensation package; including competitive hourly rate, relocation assistance, and retirement plan. To qualify you must have an AS or BS in Histology and be either HT or HTL (ASCP) certified. Working knowledge of Tissue Processors, Micrometers, Immunohistochemical and Special Staining Instrumentation is required. If you are interested in learning more about this position, please call or e-mail me at kc <@t> ka-recruiting.com

Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email with a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential. 

Histotech Openings:

* ME - Lead Surgical Pathologist

* NY - Western - Histotech

* NY - NYC - Histotech

* NYC - Pathology Manager (commercial background)

* PA - South Eastern - IHC Tech

* IN - Histotech - 2nd shift

* NC - Histology Supervisor 2nd shift

* NC - Histology Manager

* FL - Treasure Coast - Histotech

* WY - Histotech - 1st shift

* CO - Denver - Histotech

* NV - IHC tech

* CA - Southern - Histology Manager

I look forward to hearing from you.

Sincerely, 

KC Carpenter

K.A. Recruiting, Inc.

10 Post Office Square, 8th Floor South

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

kc <@t> ka-recruiting.com

www.ka-recruiting.com

------------------------------

Message: 10
Date: Thu, 3 May 2012 08:56:10 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] .._Bone Marrows
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>,	ValerieHannen
	<Valerie.Hannen <@t> parrishmed.com>
Message-ID:
	<1336060570.19838.YahooMailClassic <@t> web162105.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Our?bone marrow trephines were fixed?with neutral buffered formalin from the moment they were taken to about 6 PM (from?8 to?12 hours) in NBF before placing them in EDTA overnight.
The only thing is that we were absolutely sure that the pH of the NBF was 7.0 Always got very good results.
Ren? J.

--- On Thu, 5/3/12, Hannen, Valerie <Valerie.Hannen <@t> parrishmed.com> wrote:

From: Hannen, Valerie <Valerie.Hannen <@t> parrishmed.com>
Subject: [Histonet] .._Bone Marrows
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, May 3, 2012, 11:26 AM

Good morning Histonetters!!!

Our Pathologist has been reading again !!? He read an article about Bone Marrow fixation. I have a few questions to pose to you all.

1) Do you typically fix your Bone Marrow biopsies overnight before decalcification?? We currently do not.

2) What are you fixing them in?

Formalin?? ? ? ? Zinc Formalin? (this is what we currently are using)? ? ???B Plus? (this was the fixative mentioned in the article).

Any and all input will be greatly appreciated!!

Valerie

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com

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hereby notified that any dissemination, distribution, or
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have received this communication in error, please immediately
delete this message. Thank you"
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------------------------------

Message: 11
Date: Thu, 03 May 2012 11:19:27 -0500
From: "Brendal Finlay" <brendal.finlay <@t> medicalcenterclinic.com>
Subject: [Histonet] (no subject)
To: histonet <@t> lists.utsouthwestern.edu
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <dcf952222f6b2a74ccd276a47076b298 <@t> medicalcenterclinic.com>
Content-Type: text/plain; charset="utf-8"

We fix bone marrow cores in a B5 substitute before decalcification;
usually a minimum of 2 hours depending upon the size of the core.

??

Brendal Finlay, HT (ASCP)

------------------------------

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