[Histonet] Frozen brains on cryostat

[Boyce, Bobbie]

Hi Andrea,

I'm not sure about the unfixed tissue, but we got awesome advice from Donna Emge in regards to our wrinkle problems on fixed frozen brains.
We would dip a brush in water and run that across the slide (not too much and not too little), then pick up the tissue.
My first thought was, "Water in the cryo?", but it really worked.
Good luck!

Bobbie

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Tuesday, May 01, 2012 1:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Frozen brains on cryostat

If they haven't been fixed, good luck! I've never been able to section
unfixed tissue.
Then again, I work with embryonic tissue only.
Have you tried sectioning at a smaller section size?

Emily



The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson




On Tue, May 1, 2012 at 12:53 PM, Andrea Ferullo (non-Celgene) <
aferullo <@t> celgene.com> wrote:

> Hello everyone,
>
> I recently received rat brain samples that were frozen in liquid nitrogen
> and embedded in OCT.  I sectioned them on the cryostat and they are coming
> out very wrinkled, no matter what technique I use to pick them up.  I would
> appreciate any tips/tricks  that anyone has to offer.  Forebrain sections
> are ok, but mid, hind-brain, and cerebellum are giving me a very hard time.
>  Thanks and I look forward to your advice.
>
> Andrea
>
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