[Histonet] Phosphorylase method

[Sharon Allen]

Hi,

Does anyone have a good enzyme method for Phosphorylase done on muscle
bx's. We have been doing the method from Dubowitz, 2nd edition for years
but would like to get away from the messy PVP & coverslipping.  We are
trying cytoseal, which seems to work fine for coverslipping, but would
like to compare other methods without PVP.  Also, does anyone know the
purpose of PVP, glyceron or dextrin in this test?

Thanks for any help,

Sharon Allen

Senior Medical Technologist

Neuropathology Lab-MS435U

Health Sciences Centre

820 Sherbrook Street

Winnipeg,MB, CA 

R3A 1R9

e-mail: sallen <@t> dsmanitoba.ca

 

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