[Histonet] Intestine processing

[Bilger, Andrea]

Lyn,

If you are talking about small GI biopsies, we have a good protocol.  We have a VIP tissue processor that has  3- formalins, 1- 70% ETOH, 1- 95% ETOH, 3- 100% ETOH's, 2-xylene's and 4- paraffins on it.  Our GI biopsies are usually already fixed in formalin before we load them so we skip all the formalin steps.  We do 5 minutes in all the other stations, 70% thru paraffins.  We remove the cassettes ASAP from the processor so they do not sit in any heat for long.  This dries them out.  After embedding, blocks are faced off and allowed a brief time on ice with water on top.  These specimens are not very dry so only need a short soak before cutting any levels.  Sections are taken by cutting at a SLOW, even pace. We have been able to eliminate the "chatter" we used to see on GI biopsy slides by following this protocol.  Hope this helps.


Andrea Bilger


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