[Histonet] Problems using tomato lectin

[James S.]

Hi all,

I've just purchased Texas-Red conjugated tomato lectin from Vector and have been trying it out on some frozen mouse liver sections but cant see anything.

Protocol - fresh frozen sections 8um cut and air dried overnight. Fixed (10min) acetone, washed PBS, tomato lectin applied (I've done serial dilutions from 1/100 - 1/5000 in PBS/0.05% Tween) 1hr at room temp, wash PBS, mount Vectashield + dapi.

Any suggestions?

Thanks
Sonya