[Histonet] RE: Antiboey Diluents

[Goins, Tresa]

Not all antibody diluents contain BSA - proprietary carrier proteins include synthetic polymers.  
Not all antibody diluents are buffered with PBS - some are buffered with TBS.
If you make your antibody diluents in-house, be very selective about the BSA source, especially if you work with veterinary tissues.



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, March 21, 2012 2:43 PM
To: histonet <@t> lists.utsouthwestern.edu; Joseph Madary
Subject: Re: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS?

Joseph:
ALL commercial antibody diluent carriers  contain the same ingredients (bovine albumen, Tween 20 and sodium azide as preservative in pH7 PBS buffer).
 
Every 100 mL of antibody diluent contains:
Bovine albumen --------- 1 mL
PBS pH7 buffer --------- 99 mL
Tween 20 ----------------- 50 µL
sodium azide ----------100 mg
 
Why don't you prepare it yourself? That is what I used to do and saved a lot of money!
René J.

--- On Wed, 3/21/12, Joseph Madary <patjnm <@t> gwumc.edu> wrote:


From: Joseph Madary <patjnm <@t> gwumc.edu>
Subject: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS?
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, March 21, 2012, 11:27 AM


Hi all, I would like to get a commercially prepared antibody diluent with carrier proteins. I have been told Dako has a decent one but I am trying to find a different vendor. I see a few places with diluent but none that have background reducing substances/carrier protein type thing. 


Nick Madary, HT/HTL(ASCP)QIHC
George Washington University
Pathology Core Laboratory
Ross Hall, Room 706
23rd and I Street NW
Washington D.C. 20037
202.994.8916
patjnm <@t> gwumc.edu

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