[Histonet] questionaire about fixation

[Rene J Buesa]

Emily:
With formalin fixation, the formalin advances within the tissue in a way that you can macroscopically see up to where the formalin has penetrated. The appearance of the tissue changes in color and consistence. The change in appearance goes from the exterior to the interior of the tissue and that advance is seen as a line, hence "the front line" of the penetration.
René J.

--- On Sun, 3/18/12, Emily Sours <talulahgosh <@t> gmail.com> wrote:


From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] questionaire about fixation
To: histonet <@t> lists.utsouthwestern.edu
Date: Sunday, March 18, 2012, 10:23 PM


What the heck is a frontline?
I tried to google it, but I got nothing useful.

The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson



On Sun, Mar 18, 2012 at 2:38 PM, Gudrun Lang <gu.lang <@t> gmx.at> wrote:

> Dear histonet!
>
> A few hours after submersion of a tissue-block (eg liver) in NBF, the block
> is cut across and we see the colour-change from brown to grey at the
> margins
> until a frontline.
>
> This frontline shows us:
>
> a) the distance of penetration of NBF;
>
> b) the distance of penetration AND addition of methylol-groups;
>
> c) the distance of penetration AND addition of methylol-groups AND (at
> least
> beginning) cross-linking.
>
>
>
> Do you see the issue?
>
> I would be happy, if you can help me to find a correct answer.
>
>
>
> Regards
>
> Gudrun Lang
>
>
>
> Biomed. Analytikerin
>
> Histolab Linz, Austria
>
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