[Histonet] questionaire about fixation

[Gudrun Lang]

Dear histonet!

A few hours after submersion of a tissue-block (eg liver) in NBF, the block
is cut across and we see the colour-change from brown to grey at the margins
until a frontline.

This frontline shows us:

a) the distance of penetration of NBF; 

b) the distance of penetration AND addition of methylol-groups; 

c) the distance of penetration AND addition of methylol-groups AND (at least
beginning) cross-linking.

 

Do you see the issue?

I would be happy, if you can help me to find a correct answer.

 

Regards

Gudrun Lang

 

Biomed. Analytikerin

Histolab Linz, Austria