[Histonet] Calponin

[Mark Tarango]

I think normal breast staining of myoepithial cells is the best control for
this antibody.   We use BioGenex's antibody (product # MU333-UC) at 1:200
on the Benchmark XT (CC1 mild, 8 mins primary antibody and ultraview DAB
detection).

Mark


On Fri, Mar 16, 2012 at 7:34 AM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> DAKO monoclonal antibody at 1:1000 for 30 minutes, HIER citrate at pH6,
> colon cancer (+) case as control.
> René J.
>
> --- On Fri, 3/16/12, Chakib Boussahmain <chak_bou <@t> yahoo.com> wrote:
>
>
> From: Chakib Boussahmain <chak_bou <@t> yahoo.com>
> Subject: [Histonet] Calponin
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Friday, March 16, 2012, 9:14 AM
>
>
> Hello Histonet
> Does anyone use Calponin stain? if so can you share the protocol with us?
> What do you use for epitope retrieval?
> Many thanks in advance.
> Chakib Boussahmain
> Histotechnologist
> HTL(ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>