[Histonet] Staining GAGs in fetal membranes

[Geoff McAuliffe]

1. Try an alcoholic fixative like formalin-alcohol-acetic acid

2. Try putting Safranin O in the fixative.
Shepard and Mitchell, J. Ultrastruc. Res. 54:451-460, 1976.
or
same authors used Toluidine Blue O
J. Histochem. Cytochem. 24:621-629, 1976.

Geoff


On 3/15/2012 8:41 PM, Jennifer Scholler wrote:
> Hello,
>
> I am attempting to stain fetal membranes to look at the GAG content. My
> advisor suggested Safronin O, which I tried on a preliminary set of slides
> (hematoxylin, fast green, then safronin O). I did get some staining and saw
> a band of red staining between the layers, as expected, but the staining is
> not nearly as pronounced as in images of Safronin O stained tissues that I
> have seen (some of this obviously may be due to imaging settings). Also,
> after doing some reading, it seems that Alcian blue may be another option.
> Can anyone with experience staining FMs or similar tissues suggest a stain
> and protocol for this?
>
> Thanks!
>
> Regards,
>
> Jennifer Scholler
> M.S. candidate
> Mechanical Engineering
> University of Colorado at Boulder
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************