[Histonet] RE: Re: PAS Diastase

[Theresa (Teri) Johnson]

Tony, I agree, when looking for glycogen two patient slides should be run, one with, one without. That is not always the case. We were sometimes asked to do PAS/D on lymphomas.

Teri

-----Original Message-----
From: Tony Henwood (SCHN) [mailto:tony.henwood <@t> health.nsw.gov.au] 
Sent: Thursday, March 15, 2012 3:39 PM
To: Theresa (Teri) Johnson; histonet <@t> lists.utsouthwestern.edu
Subject: RE: Re: PAS Diastase

No,

If you need to demonstrate that PAS positive material is glycogen and not some other carbohydrate containing material you must do a PAS on the test case as well.

(see my earlier Post)

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson
Sent: Friday, 16 March 2012 3:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: PAS Diastase

I disagree that two patient slides are needed. What is needed for diagnosis is one patient slide with digestion, and two control slides, one with and one without. If the control slide shows adequate digestion of the glycogen and the other control slide shows adequate PAS positivity, the pathologist can be assured that if the patient slide has any PAS positivity it is not due to glycogen. I am guessing this is only in cases where they are not concerned with glycogen storage as part of the diagnosis (liver biopsy).

Ask your pathologist what he or she is looking for. My guess is it is not glycogen, but some other PAS positive component.



On 3/15/2012 1:47 AM, Jennifer MacDonald wrote:
> Hi All,
> At a local lab when a pathologist orders a PAS diastase the 
> histotechnicians do just one slide with diastase.  They do not do an 
> undigested slide.  How would the pathologist know if the digested 
> slide had a glycogen to begin with?  Am I over thinking this?
> Thanks,
> Jennifer
>


Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752

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