[Histonet] PAS Diastase

[Tony Henwood (SCHN)]

My two cents:

I agree as well

Though in some instances the presence of glycogen is neither here nor there. 
It might be removed just to make the diagnostic feature being sought easier to detect.

For example, we used to do routinely do AB-DiPAS on oesophageal biopsies for the detection of epithelial metaplasia as well as fungus. In this case the presence of glycogen was thought to confuse things so was removed prior to PAS staining. The presence or absence of glycogen in nearly all cases was not diagnostic. In this case an AB-PAS was not done.

Remember to look for internal controls for PAS staining (eg basement membranes).

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe
Sent: Friday, 16 March 2012 12:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Diastase

I agree with Rene. A control (undigested) slide is necessary.

Geoff

On 3/15/2012 1:47 AM, Jennifer MacDonald wrote:
> Hi All,
> At a local lab when a pathologist orders a PAS diastase the 
> histotechnicians do just one slide with diastase.  They do not do an 
> undigested slide.  How would the pathologist know if the digested 
> slide had a glycogen to begin with?  Am I over thinking this?
> Thanks,
> Jennifer
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> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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>


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
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