[Histonet] Vacuum processing question

Kara Lee karabou76 <@t> hotmail.com
Thu Mar 15 09:10:34 CDT 2012


Hello all,
I have a question about vacuum processing. 

We have ligament tissues that are processed over night, then are to be vacuum processed.  I've never done this before, but was able to get ahold of a vacuum oven.  
>From what I understand, I just put the tissues in a metal container and turn on the vacuum oven for 1 hour.  But what are they supposed to be in?  Parafin?  Alcohol?  Do I just leave them in the cassettes and not put them in any sort of liquid? 

Is there a specific temperature or vacuum that any of you have used that works particularly well?

Also, is it possible to let the tissues sit over the weekend after the regular processing before putting them in the vacuum oven for processing?

Any advice would be greatly appreciated as I am new to this world of histology, having a biochemistry background.

Many thanks in advance,
Kara
 		 	   		  


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