[Histonet] Undecalcified bone IHC

[Sean McBride]

René & Jeffery,

I don't see sectioning as that big of a challenge.  I often thin section mineralized bone sections ~7 microns with my Leica microtome.  Although I have a great interest in developing an IHC technique for mineralized bone, I have never had a spare moment to carry out any developmental work.  I am concerned more about the effects of the solvents used in hard tissue histology on the antigens/epitopes of interest.  

Does anyone use IHC staining on PMMA mineralized bone specimens on a regular basis? 


Best regards,


~Sean McBride


Scientific Specialist
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
412-915-1683 (m)
412-268-8275 (fax)
smcbride <@t> andrew.cmu.edu 






On Mar 12, 2012, at 11:06 AM, Rene J Buesa wrote:

> Undecalcified? How are you going to section it? 
> If you can section it, just use any IHC protocol for regular sections. 
> Good luck!
> René J.
> 
> --- On Mon, 3/12/12, Jeffery Howery <Jeffery.Howery <@t> jcl.com> wrote:
> 
> 
> From: Jeffery Howery <Jeffery.Howery <@t> jcl.com>
> Subject: [Histonet] Undecalcified bone IHC
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Monday, March 12, 2012, 10:59 AM
> 
> 
> Does anyone have a protocol for Undecalcified bone for IHC?
> 
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