[Histonet] ATPase reaction

[Heath, Nancy L.]

well...looks like I don't know how to attach things so they will post on
histonet...Hazel Horn I sent my procedure to your email too :)

Nancy Heath, HT (ASCP)
Neuropathology Technician
Pathology Tech Specialist
Dept. of Pathology., Div. of Neuropathology
Rhode Island Hospital
APC Blding, Flr 12, Rm 211
593 Eddy Street 
Providence, RI 02903
lab: 401-444-3246
fax: 401-444-8514
nheath <@t> lifespan.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Heath,
Nancy L.
Sent: Thursday, March 08, 2012 2:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] ATPase reaction

@ Hazel Horn...sorry about not attaching the procedure :/

Nancy Heath, HT (ASCP)
Neuropathology Technician
Pathology Tech Specialist
Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC
Blding, Flr 12, Rm 211
593 Eddy Street
Providence, RI 02903
lab: 401-444-3246
fax: 401-444-8514
nheath <@t> lifespan.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
mtitford <@t> aol.com
Sent: Thursday, March 08, 2012 7:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ATPase reaction


Hazel Horn asks about the ATPase reaction:

Years ago we had problem with our ATPase reaction on F/S of muscle
biopsies. We discovered that in keeping the substrate at minus 20oC in a
freezer, and getting it out and letting it warm up to room temperature
before weighing a small amount out for the procedure, and then putting
it back in the freezer,  in doing that all the time, we had
inadvertantly inactivated the ATPase substrate. Its best to aliquot it
out when you first receive it from Sigma, or whoever, and keep those
vials frozen, getting one out to use every time you do the reaction.

Other than that, I would think the solutions are suspect, too old or
wrong pH or something like that.

Regards
Michael Titford
Pathology  - USA
Mobile AL 
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