[Histonet] Re:Goldner's Trichrome for Osteoid

[Damien]

Hi Andi,


Yes, you can stain and differentiate osteoid using decalcified bone
sections. You can see osteoid after staining with any trichrome technique,
H&E or Toluidine blue.  The structure does not disappear with
decalcification. However, do not expect the Goldner’s stain to mimic the
staining pattern, or have the sharp contrast, that what would be observed
when using a calcified bone section. For decalcified sections, I personally
like the Gomori one step with Aniline Blue (osteoid will stain blue).



1.Yes (with some exceptions)

2. Formic acid works well

3. Try 5 microns



-Damien L.



-- 
Damien Laudier
Laudier Histology
www.LaudierHistology.com