[Histonet] Re: Histonet Digest, Vol 100, Issue 9
Miyamoto, Garret T Mr CIV USA USAMEDCOM
garret.t.miyamoto <@t> us.army.mil
Wed Mar 7 18:35:04 CST 2012
Re: SOP for release of tissues and consent form
Rhonda,
We do not have an SOP but if you have a fax, I can send you a form that we use.
Garret Miyamoto
Tripler Army Medical Center
----- Original Message -----
From: histonet-request <@t> lists.utsouthwestern.edu
Date: Wednesday, March 7, 2012 8:04 am
Subject: Histonet Digest, Vol 100, Issue 9
To: histonet <@t> lists.utsouthwestern.edu
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> Today's Topics:
>
> 1. RE: xylene substitute for processing (Marcum, Pamela A)
> 2. unsubscribe (Sparks, Joanne)
> 3. Re: RE: xylene substitute for processing
> (Grantham, Andrea L - (algranth))
> 4. Re: RE: xylene substitute for processing (Jennifer MacDonald)
> 5. modified Davidson's fixative on testes (Louro, Pedro)
> 6. Histotech Job Opening in TN-Permanent/Direct Hire (Melissa
> Phelan) 7. SOP for release of tissues & consent form (Rhonda Ford)
> 8. mesocolon lymphnode treatment with aceton (Gudrun Lang)
>
>
> -------------------------------------------------------------------
> ---
>
> Message: 1
> Date: Wed, 7 Mar 2012 15:58:13 +0000
> From: "Marcum, Pamela A" <
> Subject: [Histonet] RE: xylene substitute for processing
> To: "'Masood, Sameena Kathryn'" <,
> "Cross, Kelly" <, "Histonet Listserv (E-mail)
> (histonet <@t> lists.utsouthwestern.edu)"
> <
> Message-ID:
> <
> Content-Type: text/plain; charset="us-ascii"
>
> Some people have issues with the smell and the other option is Anatech ProPar or Richard Allan Thermo non-aliphatic hydrocarbon subs which have very low odor and are also about the same price. We can't use the orange smell in our lab as some people have allergies and others just don't like it. Both work types work very well and it is a lab choice for the options, not a quality choice. Be aware most processor companies still require xylene in the clean cycle to keep your system healthy.
>
> Pam Marcum
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Masood, Sameena Kathryn
> Sent: Wednesday, March 07, 2012 9:36 AM
> To: Cross, Kelly; Histonet Listserv (E-mail) (histonet <@t> lists.utsouthwestern.edu)
> Subject: [Histonet] RE: xylene substitute for processing
>
> Kelly,
>
> Histo-Clear can be directly substituted for Xylene at every point in processing and staining. It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also. Some guys in our Histology Core told us about it and we have been happy with it. You can purchase it from the manufacturer, National Diagnostics, or Fisher.
>
> http://www.nationaldiagnostics.com/product_info.php?products_id=175
>
> Good luck,
>
> Kathryn
>
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cross, Kelly
> Sent: Monday, March 05, 2012 10:24 AM
> To: Histonet Listserv (E-mail) (histonet <@t> lists.utsouthwestern.edu)
> Subject: [Histonet] xylene substitute for processing
>
> Greetings Histonet!
>
> Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains?
>
> Thank you in advance for your help,
> Kelly
>
> Kelly S. Cross B.S., HT (ASCP)
> Medical Laboratory Supervisor
> Veterinary Pathobiology
> Texas Veterinary Medical Center
> Texas A&M University
> College Station, TX 77843-4467
> 979-862-3658 Office
> 979-845-5149 Lab
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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>
>
>
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> ------------------------------
>
> Message: 2
> Date: Wed, 7 Mar 2012 11:09:05 -0500
> From: "Sparks, Joanne" <
> Subject: [Histonet] unsubscribe
> To: <
> Message-ID:
> <
> Content-Type: text/plain; charset="us-ascii"
>
> Please unsubscribe from histonet.
>
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 7 Mar 2012 08:12:24 -0800
> From: "Grantham, Andrea L - (algranth)" <
> Subject: Re: [Histonet] RE: xylene substitute for processing
> Cc: HISTONET <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> I have been using Clear Rite 3 for years on animal tissue in a VIP for overnite processing and it is great. I get it from Fisher. There has never been a problem with special stains but I use xylene on my stain lines.
>
> Andi Grantham
>
>
>
>
> On Mar 7, 2012, at 8:35 AM, Masood, Sameena Kathryn wrote:
>
> > Kelly,
> >
> > Histo-Clear can be directly substituted for Xylene at every point in processing and staining. It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also. Some guys in our Histology Core told us about it and we have been happy with it. You can purchase it from the manufacturer, National Diagnostics, or Fisher.
> >
> > http://www.nationaldiagnostics.com/product_info.php?products_id=175
> >
> > Good luck,
> >
> > Kathryn
> >
> >
> >
> >
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cross, Kelly
> > Sent: Monday, March 05, 2012 10:24 AM
> > To: Histonet Listserv (E-mail) (histonet <@t> lists.utsouthwestern.edu)
> > Subject: [Histonet] xylene substitute for processing
> >
> > Greetings Histonet!
> >
> > Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains?
> >
> > Thank you in advance for your help,
> > Kelly
> >
> > Kelly S. Cross B.S., HT (ASCP)
> > Medical Laboratory Supervisor
> > Veterinary Pathobiology
> > Texas Veterinary Medical Center
> > Texas A&M University
> > College Station, TX 77843-4467
> > 979-862-3658 Office
> > 979-845-5149 Lab
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> ------------------------------
>
> Message: 4
> Date: Wed, 7 Mar 2012 08:14:43 -0800
> From: Jennifer MacDonald <
> Subject: Re: [Histonet] RE: xylene substitute for processing
> To: "Marcum, Pamela A" <
> Cc: "Histonet Listserv \(E-mail\)
> \(histonet <@t> lists.utsouthwestern.edu\)"
> <, "'Masood, Sameena Kathryn'"
> <,
> histonet-bounces <@t> lists.utsouthwestern.edu, "Cross, Kelly"
> <
> Message-ID:
> <
> Content-Type: text/plain; charset="US-ASCII"
>
> One thing to take into consideration that many of the xylene substitutes
> take longer to clear than xylene. You may have to adjust your processing
> schedule. We use a xylene substitute with success.
>
>
>
>
> "Marcum, Pamela A" <
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 03/07/2012 07:58 AM
>
> To
> "'Masood, Sameena Kathryn'" <, "Cross,
> Kelly" <, "Histonet Listserv (E-mail)
> (histonet <@t> lists.utsouthwestern.edu)" <
> cc
>
> Subject
> [Histonet] RE: xylene substitute for processing
>
>
>
>
>
>
> Some people have issues with the smell and the other option is Anatech
> ProPar or Richard Allan Thermo non-aliphatic hydrocarbon subs which have
> very low odor and are also about the same price. We can't use the orange
> smell in our lab as some people have allergies and others just don't like
> it. Both work types work very well and it is a lab choice for the
> options, not a quality choice. Be aware most processor companies still
> require xylene in the clean cycle to keep your system healthy.
>
> Pam Marcum
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Masood,
> Sameena Kathryn
> Sent: Wednesday, March 07, 2012 9:36 AM
> To: Cross, Kelly; Histonet Listserv (E-mail)
> (histonet <@t> lists.utsouthwestern.edu)
> Subject: [Histonet] RE: xylene substitute for processing
>
> Kelly,
>
> Histo-Clear can be directly substituted for Xylene at every point in
> processing and staining. It's completely non-toxic (even has a food grade
> rating), is made from oranges, and smells delicious. You don't have to
> work in a hood and it seems to make tissue less brittle than Xylene also.
> Some guys in our Histology Core told us about it and we have been happy
> with it. You can purchase it from the manufacturer, National Diagnostics,
> or Fisher.
>
> http://www.nationaldiagnostics.com/product_info.php?products_id=175
>
> Good luck,
>
> Kathryn
>
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cross,
> Kelly
> Sent: Monday, March 05, 2012 10:24 AM
> To: Histonet Listserv (E-mail) (histonet <@t> lists.utsouthwestern.edu)
> Subject: [Histonet] xylene substitute for processing
>
> Greetings Histonet!
>
> Does anyone use xylene substitutes for routine over-night processing? If
> so, what do you use and does it have any adverse effect your special
> stains?
>
> Thank you in advance for your help,
> Kelly
>
> Kelly S. Cross B.S., HT (ASCP)
> Medical Laboratory Supervisor
> Veterinary Pathobiology
> Texas Veterinary Medical Center
> Texas A&M University
> College Station, TX 77843-4467
> 979-862-3658 Office
> 979-845-5149 Lab
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> is for the sole use of the intended recipient(s) and may contain
> confidential and privileged information. Any unauthorized review,
> use, disclosure or distribution is prohibited. If you are not the
> intended recipient, please contact the sender by reply
> e-mail and destroy all copies of the original message..
>
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 7 Mar 2012 11:55:26 -0500
> From: "Louro, Pedro" <
> Subject: [Histonet] modified Davidson's fixative on testes
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> Hello to all Histo techs and Happy early Histotechnology Professionals
> Day.
>
>
>
> I wanted to know if anyone out there is using modified Davidson's
> fixative to fix rat testes and staining with PAS.
>
> We have a pathologists that is looking for certain cells within the
> testes and thought that this might work.
>
>
>
> Thanks in advance for any information.
>
>
>
> Pedro Louro
>
> Technologist
>
> Histology/Immunohistochemistry
>
>
>
>
>
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> ------------------------------
>
> Message: 6
> Date: Wed, 07 Mar 2012 12:21:50 -0500
> From: Melissa Phelan <
> Subject: [Histonet] Histotech Job Opening in TN-Permanent/Direct Hire
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="US-ASCII"
>
> Hello All,
>
> I have a permanent/full time job opening for a qualified Histotech in the
> Knoxville, TN area. Please contact me for details if you are interested.
> Everyone have a great day!!!
> --
> Melissa Phelan, President Laboratory Staffing
> Allied Search Partners
> http://www.linkedin.com/in/melissaphelan
> <http://www.linkedin.com/in/melissaphelan> P: 888-388-7571
> F: 888-388-7572
> C: 407-697-1175
> www.alliedsearchpartners.com
>
>
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 7 Mar 2012 12:23:48 -0500
> From: Rhonda Ford <
> Subject: [Histonet] SOP for release of tissues & consent form
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <
> Content-Type: text/plain; charset=ISO-8859-1
>
> Does anyone have a procedure for the release of tissues along with a
> consent form that they like would like to share? Thanks in advance.
>
> --
> Rhonda Ford, Histology Lab
> Henry County Hospital
> (765) 521-1148
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 7 Mar 2012 18:24:57 +0100
> From: "Gudrun Lang" <
> Subject: [Histonet] mesocolon lymphnode treatment with aceton
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> Dear all!
>
> Does anyone out there use this method for mesocolon clearing with aceton? I
> heard only a few facts about it and would like to hear about personal
> experience.
>
> The mesocolon is first fixed in NBF, then put in pure aceton, then treated
> with a teasing roller and put in a "pipe" with holes. The tissue is
> compressed to squeeze out the fat to form a kind of sausage.
>
> The sausage is sliced up, the slices are put into cassettes and processed.
>
> I don't know the exact details. If you have the access to this article, you
> can read more (I don't have).
>
> http://journals.lww.com/ajsp/Abstract/2012/02000/Optimal_Lymph_Node_Harvest_
> in_Rectal_Cancer__UICC.5.aspx
>
> or this:
>
> http://www.springerlink.com/content/748617k331l06143/
>
>
>
> thank you
>
>
>
> Gudrun Lang
>
>
>
> Biomed. Analytikerin
>
> Histolab, Linz, Austria
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
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> End of Histonet Digest, Vol 100, Issue 9
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