[Histonet] immunofluorescence mounting medium?
Geert van Haalem
g.vanhaalem <@t> prosensa.nl
Wed Mar 7 01:54:37 CST 2012
Hi,
I have used Prolong Gold and the Vectashield with good quality images. So I doubting
whether it's the mounting media that causes the smeary effect. I could be the
washing steps in your protocol or your choice of antibodies. I would also check your
objective perhaps it has a correction ring to compensate for the refractive index.
Regards,
Geert
the Netherlands
>
> Message: 6
> Date: Mon, 5 Mar 2012 09:33:38 -0800
> From: "Collette, Nicole M." <collette2 <@t> llnl.gov>
> Subject: [Histonet] immunofluorescence mounting medium?
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <CB7A38F2.6775%collette2 <@t> llnl.gov>
> Content-Type: text/plain; charset="Windows-1252"
>
> Hello, Esteemed Histonetters,
>
> I am trying to get nice publication-quality images of my immunofluorescent
> tissue sections. I am currently using Prolong Gold, and after I let the
> stuff cure for several days, my 100X oil-immersion images are still smeary,
> even after taking into account the unevenness of the tissue section. I
> don't seem to have this problem with colorimetric stains (histological
> stains, LacZ, etc.), so I am thinking the mounting medium is part of the
> problem? It seems to me that it never fully cures at the middle of the
> coverslip? Does anyone have a recommendation for a mounting medium that
> works better for this purpose? Any advice is appreciated.
>
> Thanks in advance, and Happy Monday!
>
> Sincerely,
> Nicole Collette
> Lawrence Livermore National Laboratory
> collette2 <@t> llnl.gov
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 5 Mar 2012 17:40:26 +0000
> From: "Wen,Yujie" <yujie.wen <@t> louisville.edu>
> Subject: RE: [Histonet] immunofluorescence mounting medium?
> To: "Collette, Nicole M." <collette2 <@t> llnl.gov>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <wjwpwfroqwsqehu6dn2jl2tp.1330969083131 <@t> email.android.com>
> Content-Type: text/plain; charset="Windows-1252"
>
> http://www.vectorlabs.com/catalog.aspx?catID=279
>
> VECTASHIELD Mounting Media for Fluorescence
>
> This one works for me and was recommended by Leica technical support for
> oil immersion image.
>
> -------- Original Message --------
> From: Collette, Nicole M.
> Sent: Mon, Mar 5, 2012 12:35 PM
> To: histonet <@t> lists.utsouthwestern.edu
> CC:
> Subject: [Histonet] immunofluorescence mounting medium?
>
>
> Hello, Esteemed Histonetters,
>
> I am trying to get nice publication-quality images of my immunofluorescent
> tissue sections. I am currently using Prolong Gold, and after I let the
> stuff cure for several days, my 100X oil-immersion images are still smeary,
> even after taking into account the unevenness of the tissue section. I
> don't seem to have this problem with colorimetric stains (histological
> stains, LacZ, etc.), so I am thinking the mounting medium is part of the
> problem? It seems to me that it never fully cures at the middle of the
> coverslip? Does anyone have a recommendation for a mounting medium that
> works better for this purpose? Any advice is appreciated.
>
> Thanks in advance, and Happy Monday!
>
> Sincerely,
> Nicole Collette
> Lawrence Livermore National Laboratory
> collette2 <@t> llnl.gov
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 100, Issue 5
> ****************************************
>
--
Charity Wyatt
Mohs Histotechnologist
Jacksonville Skin Cancer Center, P.A./
Michael E. Lutz, M.D.
(904)737-0111
------------------------------
Message: 7
Date: Mon, 5 Mar 2012 15:39:28 -0500
From: "Virginia Chladek" <virginiachladek <@t> wpuafl.com>
Subject: [Histonet] Destain/Restain
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9BB0A278800B0E41BBC28883FFAB45357DE363 <@t> exchsvr.wpu.local>
Content-Type: text/plain; charset="us-ascii"
****************************************
I need your help-
My doc needs me to destain an h&e slide to run a ck5 on the Bond3, how
can I do this without the tissue falling off? I ran the slide back down
from xylene to water and destained, then introduced the slide to bond
wash before starting the protocol in bond wash again and lost the
tissue. Any suggestions?
Thanks!
Maggie
------------------------------
Message: 8
Date: Mon, 5 Mar 2012 12:52:18 -0800 (PST)
From: Kim Tournear <kim.tournear <@t> yahoo.com>
Subject: [Histonet] histology programs in Okalahoma
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1330980738.90192.YahooMailNeo <@t> web120205.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi everyone,
Does anyone know of any histology training/programs in Oklahoma?
Thanks in advance for all responses.
~Kim~?
OU ROCKS!!!!
~Don't be afraid your life will end,
be afraid it will never begin~
------------------------------
Message: 9
Date: Mon, 5 Mar 2012 16:27:54 -0600
From: "Mitchell Jean A" <JMitchell <@t> uwhealth.org>
Subject: [Histonet] 2012 Tri-State Symposium
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AF5FFDEFFA2ECA4581BF6115DF17E456871036 <@t> UWHC-MAIL4.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Dear Histonetters: You are invited to join the histology societies of
Wisconsin, Iowa and Minnesota as they host "An Ocean of Knowledge" at
the 2012 Tri-State Symposium, May 2-4 at The Concourse Hotel in Madison,
Wisconsin.
For program, registration and vendor/exhibit information contact the
following representatives:
Wisconsin: Jean Mitchell (jmitchell <@t> uwhealth.org)
Iowa: Judi Stasko (judith.stasko <@t> ars.usda.gov)
Minnesota: Lois Rowe (rowe.lois <@t> mayo.edu)
Vendor/Exhibit: Dawn Schneider (dawn.schneider <@t> ministryhealth.org)
------------------------------
Message: 10
Date: Mon, 5 Mar 2012 19:43:44 -0500
From: Komal Gada <kjgada <@t> gmail.com>
Subject: [Histonet] Question about slides
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CABhfMa6PAe6TsqLax6S0eKUY0e38-GPDLcrpgJH_9z=NrfzD3g <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello Histo-netters,
I'm looking for a source which sells slides for a Tissue Identification
class. Does anyone have any leads on where to go for something like this?
Thanks,
Komal
------------------------------
Message: 11
Date: Mon, 5 Mar 2012 16:45:35 -0800 (PST)
From: Tuyen Nguyen <tuyenmai77 <@t> yahoo.com>
Subject: [Histonet] Greetings, friend!
To: no-reply-37454207n00 <@t> flickr.com, lechi1962 <@t> yahoo.com,
minhnguyet1818 <@t> yahoo.com, histonet <@t> lists.utsouthwestern.edu,
plucas <@t> biopath.org, flastname <@t> aerotek.com,
svanlancker <@t> memorialcare.org
Message-ID:
<1330994735.93267.yint-ygo-j2me <@t> web162803.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=us-ascii
http://extendiendomanos.org/like.php?ficosyzycuge=38&qiqeli=625&jrexesofy=78
------------------------------
Message: 12
Date: Mon, 5 Mar 2012 17:32:32 -0800 (PST)
From: angela smith <we3smitty <@t> yahoo.com>
Subject: [Histonet] glacial acetic acid vs bouins
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1330997552.1563.YahooMailClassic <@t> web125401.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this.?
Does anyone out there remember what dilution works best??
Thanks
------------------------------
Message: 13
Date: Tue, 6 Mar 2012 07:49:16 +0000
From: Jonathan Cremer <Jonathan.Cremer <@t> med.kuleuven.be>
Subject: RE: [Histonet] glacial acetic acid vs bouins
To: angela smith <we3smitty <@t> yahoo.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C35C2709C22FC2458D6A918FACC338A301588C <@t> ICTS-S-MBX5.luna.kuleuven.be>
Content-Type: text/plain; charset="us-ascii"
Just an acid wash then, as in the wash after the methyl blue in an MSB or Mallory's trichrome? I have 1% and 0.5% for those stains, respectively. My guess would be that the concentration of acetic acid is not that important, just the fact that it lowers the pH of the water so specific stain does not wash out of the section.
No idea what stain you're doing though. Might be something else entirely.
Best
---
Jonathan Cremer
Laboratory Technician
TARGID - KU Leuven
________________________________________
Van: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] namens angela smith [we3smitty <@t> yahoo.com]
Verzonden: dinsdag 6 maart 2012 2:32
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: [Histonet] glacial acetic acid vs bouins
I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this.
Does anyone out there remember what dilution works best?
Thanks
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Tue, 6 Mar 2012 06:35:33 -0600
From: "Giroux, Stacy" <Stacy.Giroux <@t> stjohn.org>
Subject: [Histonet] Smad4 (B-8) / DPC4
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<29CCF3EC44815745864D9F84A1ED4B51B3E0709AE9 <@t> AUSP03VMBX11.apptixhealth.net>
Content-Type: text/plain; charset="iso-8859-1"
Hi,
I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are currently trying to work up this antibody for validation; however, we are having significant problems getting it to work consistently. We are using the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If anyone has protocol information to share regardless of platform used it would be greatly appreciated.
Thanks,
Stacy
Stacy Giroux, HTL(ASCP)
Operations Coordinator, Histology
St. John Hospital & Medical Center
Phone: 313-343-3130
Fax: 313-343-4965
Histotechnology Professionals Day - March 10, 2012
CONFIDENTIALITY NOTICE:
This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege.
------------------------------
Message: 15
Date: Tue, 6 Mar 2012 08:31:52 -0500
From: "Gill, Caula A." <cgill <@t> marylandgeneral.org>
Subject: RE: [Histonet] glacial acetic acid vs bouins
To: "angela smith" <we3smitty <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<087A9911BBAFDE4B8151CB148586E2C23AA011 <@t> MDGEN-EXCH1.marylandgeneral.org>
Content-Type: text/plain; charset="iso-8859-1"
What works best for us is a 10% acetic acid solution. We mix up a gallon whenever needed and dip large tissues in the solution. For smaller sections of tissue a squirt bottle works well.
Hope this helps .
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of angela smith
Sent: Monday, March 05, 2012 8:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] glacial acetic acid vs bouins
I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this. Does anyone out there remember what dilution works best??
Thanks
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Tue, 06 Mar 2012 08:34:00 -0500
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: Re: [Histonet] Smad4 (B-8) / DPC4
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Stacy Giroux"
<Stacy.Giroux <@t> stjohn.org>
Message-ID: <4F55CBF8.2B7F.00C9.1 <@t> geisinger.edu>
Content-Type: text/plain; charset="us-ascii"
I've tried to work it up on the XT also with inconsistent, weak results. If you get a reply on this one, let me know.
Angie
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
>>> "Giroux, Stacy" <Stacy.Giroux <@t> stjohn.org> 3/6/2012 7:35 AM >>>
Hi,
I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are currently trying to work up this antibody for validation; however, we are having significant problems getting it to work consistently. We are using the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If anyone has protocol information to share regardless of platform used it would be greatly appreciated.
Thanks,
Stacy
Stacy Giroux, HTL(ASCP)
Operations Coordinator, Histology
St. John Hospital & Medical Center
Phone: 313-343-3130
Fax: 313-343-4965
Histotechnology Professionals Day - March 10, 2012
CONFIDENTIALITY NOTICE:
This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail.
-------------- next part --------------
BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Bitting, Angela
TEL;WORK:570-271-6844
ORG:;Histology
EMAIL;WORK;PREF:AKBITTING <@t> geisinger.edu
N:Bitting;Angela
END:VCARD
------------------------------
Message: 17
Date: Tue, 6 Mar 2012 08:34:25 -0500
From: "Gill, Caula A." <cgill <@t> marylandgeneral.org>
Subject: RE: [Histonet] glacial acetic acid vs bouins
To: "angela smith" <we3smitty <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<087A9911BBAFDE4B8151CB148586E2C23AA014 <@t> MDGEN-EXCH1.marylandgeneral.org>
Content-Type: text/plain; charset="iso-8859-1"
What works best for us is a 10% acetic acid solution. We mix up a gallon whenever needed and dip large tissues in the solution. For smaller sections of tissue a squirt bottle works well.
Hope this helps .
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of angela smith
Sent: Monday, March 05, 2012 8:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] glacial acetic acid vs bouins
I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this. Does anyone out there remember what dilution works best??
Thanks
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Tue, 6 Mar 2012 08:46:49 -0500
From: John Baker <bakerj <@t> umich.edu>
Subject: [Histonet] tissue processors
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <6274951A-A955-43AF-A745-9CD70ED76F23 <@t> umich.edu>
Content-Type: text/plain; charset=us-ascii
Hello Histoworld, What processors are people using to process bone or soft tissue samples for plastic? Do any of your processors allow for use of the monomer in the processor before embedding? What are your opinions, pro or cons, on the Tissue- Tek VIP5 or 6, the Leica ASP300, or the Thermo Pathcentre units? Hearing yours experiences in using them is greatly appreciated! My best, John
John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200
734-936-1635
------------------------------
Message: 19
Date: Tue, 6 Mar 2012 14:01:49 +0000
From: "Chiriboga, Luis" <Luis.Chiriboga <@t> nyumc.org>
Subject: [Histonet] IHC + control question
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3E6798F00C9F494399E96B720ECD1429C76E <@t> MSGWCDCPMB22.nyumc.org>
Content-Type: text/plain; charset="us-ascii"
Posting for a colleague:
Which of the following choices
Cytokeratin (NOS)
Vimentin
s-100
Cd45
Desmin
Would be the best to use as an internal positive control for fixation and processing?
Any supporting literature/references/docuemntation would be very helpful...
Thanks
Luis
------------------------------
Message: 20
Date: Tue, 6 Mar 2012 09:04:10 -0500
From: John Shelley <jshelley <@t> sanfordburnham.org>
Subject: [Histonet] CD11c and CD206
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5A605CE38EECB64B94485C02125A0C441AB810B061 <@t> LN-MAIL07.ln.burnham.org>
Content-Type: text/plain; charset="iso-8859-1"
Hi All,
Was wondering if anyone has had experince using these antibodies. I have been given these antibodies from an investigator and they are from Ab Serotec. One is Rabbit and the other is Armenian Hamster it appears I can get the CD11c to work with a mild protease but the CD 206 does not appear to work under all the conditions I have tried. Too many to list but If someone has worked with both or one of these antibodies your help would be greatly appreciated.
Kind regards!
John Shelley
------------------------------
Message: 21
Date: Tue, 6 Mar 2012 07:42:54 -0700
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Control Block Tracking
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<02C099024072804EA34F5906BAC30A413F1F5A <@t> nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
During a recent inspection of my department, the inspector "encouraged"
me to develop a method of tracking which control tissue I used for a
case (i.e., individually identifying the block and referencing that
identifier on the slide). To be honest, I believe this to be overload,
but since I'm probably going to have to abide by that "encouragement",
I'd like to know if/how you identify which control block is used for a
special stain. I can think of a simple method but wonder if any of you
have used a really spectacular method that just blew the inspecting
agency away. I'd promptly plagiarize it! Gracias!
Sally Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
1101 Camino de Salud NE
Albuquerque, NM 87102
505-383-9278 (Histology Lab)
------------------------------
Message: 22
Date: Tue, 6 Mar 2012 09:57:22 -0500
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: [Histonet] RE: Destain/Restain
To: 'Virginia Chladek' <virginiachladek <@t> wpuafl.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E9A90E28259D2F4E84308C5E8EA8F7B4B8D5539A4A <@t> lmhs-exchange.lmhealth.org>
Content-Type: text/plain; charset="us-ascii"
We do not destain h&e slides. We just take them back to water and stain right over top of the h&e.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Virginia Chladek
Sent: Monday, March 05, 2012 3:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Destain/Restain
****************************************
I need your help-
My doc needs me to destain an h&e slide to run a ck5 on the Bond3, how
can I do this without the tissue falling off? I ran the slide back down
from xylene to water and destained, then introduced the slide to bond
wash before starting the protocol in bond wash again and lost the
tissue. Any suggestions?
Thanks!
Maggie
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
------------------------------
Message: 23
Date: Tue, 06 Mar 2012 10:04:12 -0500
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: Re: [Histonet] IHC + control question
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Luis Chiriboga"
<Luis.Chiriboga <@t> nyumc.org>
Message-ID: <4F55E11D.2B7F.00C9.1 <@t> geisinger.edu>
Content-Type: text/plain; charset=US-ASCII
My vote has always been for vimentin. See Dako's booklet IHC Staining Methods, 5th Ed, Chapter 19 addresses this.
Angie
>>> "Chiriboga, Luis" <Luis.Chiriboga <@t> nyumc.org> 3/6/2012 9:01 AM >>>
Posting for a colleague:
Which of the following choices
Cytokeratin (NOS)
Vimentin
s-100
Cd45
Desmin
Would be the best to use as an internal positive control for fixation and processing?
Any supporting literature/references/docuemntation would be very helpful...
Thanks
Luis
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail.
------------------------------
_______________________________________________
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End of Histonet Digest, Vol 100, Issue 6
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