[Histonet] IHC + control question

[Richard Cartun]

I know that many labs run "Vimentin" for this, but please give consideration to running a nuclear marker as well especially since some of our predictive markers (ER and PR) are nuclear proteins.  In my opinion, Vimentin is very "robust" and may show-up even when the tissue is not adequately fixed and/or processed.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Chiriboga, Luis" <Luis.Chiriboga <@t> nyumc.org> 3/6/2012 9:01 AM >>>
Posting for a colleague:

Which of the following choices

Cytokeratin (NOS)
Vimentin
s-100
Cd45
Desmin

Would be the best to use as an internal positive control for fixation and processing?
Any supporting literature/references/docuemntation would be very helpful...

Thanks
Luis
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