[Histonet] glacial acetic acid vs bouins

[Jonathan Cremer]

Just an acid wash then, as in the wash after the methyl blue in an MSB or Mallory's trichrome? I have 1% and 0.5% for those stains, respectively. My guess would be that the concentration of acetic acid is not that important, just the fact that it lowers the pH of the water so specific stain does not wash out of the section.
No idea what stain you're doing though. Might be something else entirely.

Best
---
Jonathan Cremer
Laboratory Technician
TARGID - KU Leuven


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Van: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] namens angela smith [we3smitty <@t> yahoo.com]
Verzonden: dinsdag 6 maart 2012 2:32
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: [Histonet] glacial acetic acid vs bouins

I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this.
Does anyone out there remember what dilution works best?

Thanks
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