[Histonet] RE: xylene substitute for processing

[Vanessa Avalos]

I use the Shandon Excelsior and use an overnight run with Iso Alcohol and only use Xylene in the cleaning cycle. Our Thermo Rep came out and set it all up for us. No problems with the processing. I also use sub xylene in staining with a few extra steps but no problems. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cross, Kelly
Sent: Monday, March 05, 2012 9:24 AM
To: Histonet Listserv (E-mail) (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] xylene substitute for processing

Greetings Histonet!

Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains?

Thank you in advance for your help,
Kelly

Kelly S. Cross B.S., HT (ASCP)
Medical Laboratory Supervisor
Veterinary Pathobiology
Texas Veterinary Medical Center
Texas A&M University
College Station, TX 77843-4467
979-862-3658 Office
979-845-5149 Lab

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