AW: [Histonet] microtomy

[Kim Donadio]

We are talking about reality here. Not what it says in some text book written by someone who hasn't cut a block in years.

The tech is going to take the first good looking section they get after they have sat on the ice most Likely waiting for the tech who is so busy with 100 other task. 

So if long sitting bloated blocks is a new practice in your daily routine. Sure. You can get some thick sections tossed in your days work. 

Happy days. 

Sent from my iPhone

On Mar 3, 2012, at 8:39 AM, "Gudrun Lang" <gu.lang <@t> gmx.at> wrote:

> I thought it as a rule, that the thickness of slides vary depending on
> everything (inclusive weather, humidity, person, speed, temperature, cutting
> angle...).
> Although not very scientific, only the techs experience and "eye" will fix
> the problem.
> 
> As for the duration on ice: If you have "wet" ice, the tissue can take up
> the humidiy and swell (if too long, the tissue comes beyond the surface),
> but the slides wouldn't be thicker. The cooler the block, the longer the
> temperature is held while sectioning, the longer the thickness is stable.
> But if you give one breath to the surface, the section is possibly double
> thick.
> 
> Gudrun
> 
> -----Ursprüngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Webb,
> Dorothy L
> Gesendet: Freitag, 02. März 2012 19:07
> An: 'histonet <@t> lists.utsouthwestern.edu'
> Betreff: [Histonet] microtomy
> 
> We have run into an interesting scenario and wondering what the "experts"
> think!  We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on
> one particular microtome.  Within the past month, the hematopathologist has
> felt the sections are thicker than the usual 3 microns.  I had our service
> technician  measure the microns and the equipment was cutting as set.  I had
> the blocks cut on a different microtome and we have seen variations there
> also.  My question is, does the amount of time on ice make a minor
> difference in the section thickness?  I know a lot of responses may be the
> difference in the tech cutting inasmuch as how fast they turn the rotations,
> etc., but,we have ruled out that variable by having more than one tech cut
> at the microtome in question. I am stymied as to how to remedy this
> fluctuation!  This is why we love histology, so many variables to create a
> problem and why I love histonet, so many techs to help one through a
> dilemma!!  Thank you!!
> 
> Dorothy Webb, HT (ASCP)
> 
> 
> 
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