[Histonet] blocking endogenous peroxidase

[Anatoli Gleiberman]

Some peroxidase-like enzymes in macrophages (probably catalase) and DAB-binding activities in erythrocytes (hemoglobin) are not blocked by formalin. The best way to block this activity was incubation with methanol-peroxide (99 parts of methanol-1 part of 30% peroxide) during de-waxing. 3% peroxide is also effective, but in some cases it causes extensive bubbling that can damage fragile sections. So, I am using methanol-peroxide for paraffin sections and stable peroxide buffer (buffer provided in DAB developer kits, such as Pierce metal-enhanced DAB kit) for cryo- sections.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs<mailto:AGleiberman <@t> cbiolabs>.com


 


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