[Histonet] Congo red

[Bryan Llewellyn]

Bennhold's method is not the easiest to give good results. Try 
Highmans's method 
(http://stainsfile.info/StainsFile/stain/amyloid/congohighman.htm). It 
is much easier to control.

Remember that congo red is not an intensely coloured dye and the results 
are often pale. If your pathologists want a punchier stain try sirius 
red F3B (NOT 4B), as this is a deeper red than congo red. It can be used 
in a Highman type procedure as a direct substitute for congo red. It 
also gives green birefringence, again somewhat darker than congo red.

Bryan Llewellyn



Cheri Miller wrote:
> Anyone have any solution to a week Congo Red? I use Rowley  Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak.
>
> Cheryl A. Miller HT(ASCP)cm
> Histology/Cytology Prep Supervisor
> Physicians Laboratory Services
> Omaha, NE. 402 731 4145 ext. 554
>
>
> ________________________________
> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>