[Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates

[Bea DeBrosse-Serra]

We use Jackson Immunoresearch for our secondaries and the Biocare Alk. Phosph. Kit or the DAKO DAB plus kit.

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick
Sent: Wednesday, June 27, 2012 9:45 AM
To: 'Histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates

Hi everyone,

I have been using DAKO Kits for HRP anti rabbit and HRP anti Mouse and AEC substrates.

I love DAKO, but their kits and reagents are expensive.

Can anyone offer an alternative for HRP labeled anti (Rabbit/Mouse) antibodies and AEC substrate kits.

I do all my IHC manually by hand.  I am doing my IHCs primarily on FFPE slides of various tissues, some of which are large enough that they take up a lot of the slide, and hence need more drops to get complete coverage.

Thanks

Patrick.

PS: I am considering Vector Labs, and I am also looking at Biocompare to seek out more vendors.
      Has anyone tried Spring Bioscience's products?




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