[Histonet] Luxol Fast Blue problem

Houston, Ronald Ronald.Houston <@t> nationwidechildrens.org
Wed Jun 27 12:17:36 CDT 2012

Luxol fast blue staining is dependent on the polarity of the solvent. Weak staining can occur if the solvent becomes too polar, i.e. if sections are taken down to water rather than 95% alcohol prior to putting in the staining solution. Also check the amount of water in the ethanol or methanol used to make up the LFB

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim Wheelock
Sent: Wednesday, June 27, 2012 11:10 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Luxol Fast Blue problem

Hi again all:

Lately, I have had problems with my Luxol Fast Blue myelin stain not being nearly as dark as it should.
I am having my oven checked out  because  I have had problems with  it holding a  high enough temperature (at least 60C).
This seems to one factor in getting the stain to take.
But I suspect there may be other factors involved.
Has anyone had this sort of problem before? What did you find was the cause and how did you fix the problem?

Thanks for any advice you can provide.

Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont, MA

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