[Histonet] Elastic Stain

Amos Brooks amosbrooks <@t> gmail.com
Thu Jun 21 16:47:18 CDT 2012


Hi Dorothy,
     Controls for Verhoeff vanGieson elastic stain is very subjective due
to the differentiation in ferric chloride. Large arteries are usually used
as a control. If you differentiate all the slides based upon the appearance
of a large artery the small vessels will be totally gone. If you use a
small vessel as a control you will have underdifferentiated large artery.
Every slide should be differentiated individually for optimal results. Yes,
it is laborious, but the end result is well worth it.

All the best,
Amos


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